Quillaja brasiliensis saponins induce robust humoral and cellular responses in a bovine viral diarrhea virus vaccine in mice

被引：22

作者：

Cibulski, Samuel Paulo ^{[1
,2
]}

Silveira, Fernando ^{[3
]}

Mourglia-Ettlin, Gustavo ^{[4
]}

Teixeira, Thais Fumaco ^{[1
,2
]}

dos Santos, Helton Fernandes ^{[1
,2
]}

Yendo, Anna Carolina ^{[5
,6
]}

de Costa, Fernanda ^{[5
,6
]}

Fett-Neto, Arthur Germano ^{[5
,6
]}

Gosmann, Grace ^{[7
]}

Roehe, Paulo Michel ^{[1
,2
]}

机构：

[1] Inst Pesquisas Vet Desiderio Finamor, Lab Virol, FEPAGRO Saude Anim, Eldorado Do Sul, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Dept Microbiol Imunol & Parasitol, Virol Lab, BR-90610000 Porto Alegre, RS, Brazil

[3] Univ Republ UdelaR, Fac Med, Dept Desarrollo Biotecnol, Lab Carbohidratos & Glicoconjugados, Ave Alfredo Navarro 3051, Montevideo 11600, Uruguay

[4] Univ Republ UdelaR, Fac Ciencias Quim, Catedra Inmunol, Dept Biociencias, Ave Alfredo Navarro 3051, Montevideo 11600, Uruguay

[5] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Lab Fisiol Vegetal, BR-90610000 Porto Alegre, RS, Brazil

[6] Univ Fed Rio Grande do Sul, Dept Bot, BR-90610000 Porto Alegre, RS, Brazil

[7] Univ Fed Rio Grande do Sul, Fac Farm, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2016年 / 45卷

关键词：

Vaccine; Adjuvant; Quillaja brasiliensis; QB-90; BVDV; Immune response; Cellular immunity; ADJUVANTS; INFECTIONS;

D O I：

10.1016/j.cimid.2016.01.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A saponin fraction extracted from Quillaja brasiliensis leaves (QB-90) and a semi-purified aqueous extract (AE) were evaluated as adjuvants in a bovine viral diarrhea virus (BVDV) vaccine in mice. Animals were immunized on days 0 and 14 with antigen plus either QB-90 or AE or an oil-adjuvanted vaccine. Two-weeks after boosting, antibodies were measured by ELISA; cellular immunity was evaluated by DTH, lymphoproliferation, cytokine release and single cell IFN-gamma production. Serum anti-BVDV IgG, IgG1 and IgG2b were significantly increased in QB-90- and AE-adjuvanted vaccines. A robust DTH response, increased splenocyte proliferation, Th1-type cytokines and enhanced production of IFN-gamma by CD4(+) and CD8(+) T lymphocytes were detected in mice that received QB-90-adjuvanted vaccine. The AE-adjuvanted preparation stimulated humoral responses but not cellular immune responses. These findings reveal that QB-90 is capable of stimulating both cellular and humoral immune responses when used as adjuvant. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1 / 8

页数：8

共 50 条

[11] Electroporation enhances immune responses and protection induced by a bovine viral diarrhea virus DNA vaccine in newborn calves with maternal antibodies
Littel-van den Hurk, Sylvia van Drunen
Lawman, Zoe
Wilson, Don
Luxembourg, Alain
Ellefsen, Barry
van den Hurk, Jan V.
Hannaman, Drew
VACCINE, 2010, 28 (39) : 6445 - 6454
[12] Baculovirus Display of Varicella-Zoster Virus Glycoprotein E Induces Robust Humoral and Cellular Immune Responses in Mice
Xue, Wenhui
Li, Tingting
Zhang, Sibo
Wang, Yingbin
Hong, Minqing
Cui, Lingyan
Wang, Hong
Zhang, Yuyun
Chen, Tingting
Zhu, Rui
Chen, Zhenqin
Zhou, Lizhi
Zhang, Rongwei
Cheng, Tong
Zheng, Qingbing
Zhang, Jun
Gu, Ying
Xia, Ningshao
Li, Shaowei
VIRUSES-BASEL, 2022, 14 (08):
[13] Effects of injectable trace minerals on humoral and cell-mediated immune responses to Bovine viral diarrhea virus, Bovine herpes virus 1 and Bovine respiratory syncytial virus following administration of a modified-live virus vaccine in dairy calves
Palomares, R. A.
Hurley, D. J.
Bittar, J. H. J.
Saliki, J. T.
Woolums, A. R.
Moliere, F.
Havenga, L. J.
Norton, N. A.
Clifton, S. J.
Sigmund, A. B.
Barber, C. E.
Berger, M. L.
Clark, M. J.
Fratto, M. A.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2016, 178 : 88 - 98
[14] Novel ISCOMs from Quillaja brasiliensis saponins induce mucosal and systemic antibody production, T-cell responses and improved antigen uptake
Cibulski, Samuel Paulo
Mourglia-Ettlin, Gustavo
Teixeira, Thais Fumaco
Quirici, Lenora
Roehe, Paulo Michel
Ferreira, Fernando
Silveira, Fernando
VACCINE, 2016, 34 (09) : 1162 - 1171
[15] Anti-bovine herpesvirus and anti-bovine viral diarrhea virus antibody responses in pregnant Holstein dairy cattle following administration of a multivalent killed virus vaccine
Smith, Billy I.
Rieger, Randall H.
Dickens, Charlene M.
Schultz, Ronald D.
Aceto, Helen
AMERICAN JOURNAL OF VETERINARY RESEARCH, 2015, 76 (10) : 913 - 920
[16] Novel bovine viral diarrhea virus (BVDV) virus-like particle vaccine candidates presenting the E2 protein using the SpyTag/SpyCatcher system induce a robust neutralizing antibody response in mice
Katsura, Miki
Fukushima, Masaki
Kameyama, Ken-ichiro
Kokuho, Takehiko
Nakahira, Yoichi
Takeuchi, Kaoru
ARCHIVES OF VIROLOGY, 2023, 168 (02)
[17] Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus
Bálint, A
Baule, C
Pálfi, V
Belák, S
VETERINARY RESEARCH, 2005, 36 (01) : 89 - 99
[18] IMXQB-80: A Quillaja brasiliensis saponin-based nanoadjuvant enhances Zika virus specific immune responses in mice
Cibulski, Samuel
Teixeira, Thais Fumaco
Muterle Varela, Ana Paula
de Lima, Matheus Fabiao
Casanova, Gabriela
Nascimento, Yuri Mangueira
Tavares, Josean Fechine
da Silva, Marcelo Sobral
Sesterheim, Patricia
Souza, Diogo Onofre
Roehe, Paulo Michel
Silveira, Fernando
VACCINE, 2021, 39 (03) : 571 - 579
[19] DNA vaccination against bovine viral diarrhoea virus induces humoral and cellular responses in cattle with evidence for protection against viral challenge
Nobiron, I
Thompson, I
Brownlie, J
Collins, ME
VACCINE, 2003, 21 (17-18) : 2082 - 2092
[20] Fetal Lymphoid Organ Immune Responses to Transient and Persistent Infection with Bovine Viral Diarrhea Virus
Knapek, Katie J.
Georges, Hanah M.
Van Campen, Hana
Bishop, Jeanette, V
Bielefeldt-Ohmann, Helle
Smirnova, Natalia P.
Hansen, Thomas R.
VIRUSES-BASEL, 2020, 12 (08):

← 1 2 3 4 5 →