Quillaja brasiliensis saponins induce robust humoral and cellular responses in a bovine viral diarrhea virus vaccine in mice

被引：22

作者：

Cibulski, Samuel Paulo ^{[1
,2
]}

Silveira, Fernando ^{[3
]}

Mourglia-Ettlin, Gustavo ^{[4
]}

Teixeira, Thais Fumaco ^{[1
,2
]}

dos Santos, Helton Fernandes ^{[1
,2
]}

Yendo, Anna Carolina ^{[5
,6
]}

de Costa, Fernanda ^{[5
,6
]}

Fett-Neto, Arthur Germano ^{[5
,6
]}

Gosmann, Grace ^{[7
]}

Roehe, Paulo Michel ^{[1
,2
]}

机构：

[1] Inst Pesquisas Vet Desiderio Finamor, Lab Virol, FEPAGRO Saude Anim, Eldorado Do Sul, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Dept Microbiol Imunol & Parasitol, Virol Lab, BR-90610000 Porto Alegre, RS, Brazil

[3] Univ Republ UdelaR, Fac Med, Dept Desarrollo Biotecnol, Lab Carbohidratos & Glicoconjugados, Ave Alfredo Navarro 3051, Montevideo 11600, Uruguay

[4] Univ Republ UdelaR, Fac Ciencias Quim, Catedra Inmunol, Dept Biociencias, Ave Alfredo Navarro 3051, Montevideo 11600, Uruguay

[5] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Lab Fisiol Vegetal, BR-90610000 Porto Alegre, RS, Brazil

[6] Univ Fed Rio Grande do Sul, Dept Bot, BR-90610000 Porto Alegre, RS, Brazil

[7] Univ Fed Rio Grande do Sul, Fac Farm, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2016年 / 45卷

关键词：

Vaccine; Adjuvant; Quillaja brasiliensis; QB-90; BVDV; Immune response; Cellular immunity; ADJUVANTS; INFECTIONS;

D O I：

10.1016/j.cimid.2016.01.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A saponin fraction extracted from Quillaja brasiliensis leaves (QB-90) and a semi-purified aqueous extract (AE) were evaluated as adjuvants in a bovine viral diarrhea virus (BVDV) vaccine in mice. Animals were immunized on days 0 and 14 with antigen plus either QB-90 or AE or an oil-adjuvanted vaccine. Two-weeks after boosting, antibodies were measured by ELISA; cellular immunity was evaluated by DTH, lymphoproliferation, cytokine release and single cell IFN-gamma production. Serum anti-BVDV IgG, IgG1 and IgG2b were significantly increased in QB-90- and AE-adjuvanted vaccines. A robust DTH response, increased splenocyte proliferation, Th1-type cytokines and enhanced production of IFN-gamma by CD4(+) and CD8(+) T lymphocytes were detected in mice that received QB-90-adjuvanted vaccine. The AE-adjuvanted preparation stimulated humoral responses but not cellular immune responses. These findings reveal that QB-90 is capable of stimulating both cellular and humoral immune responses when used as adjuvant. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1 / 8

页数：8

共 25 条

[11] Cytotoxic T lymphocyte activation by cross-priming [J].

Heath, WR ;

Carbone, FR .

CURRENT OPINION IN IMMUNOLOGY, 1999, 11 (03) :314-318

[12] LYMPHOCYTE-MEDIATED CYTOTOXICITY IN-VITRO AND IN-VIVO - MECHANISMS AND SIGNIFICANCE [J].

KAGI, D ;

LEDERMANN, B ;

BURKI, K ;

ZINKERNAGEL, RM ;

HENGARTNER, H .

IMMUNOLOGICAL REVIEWS, 1995, 146 :95-115

[13] Beyond adjuvants: Immunomodulation strategies to enhance T cell immunity [J].

Kamphorst, Alice O. ;

Araki, Koichi ;

Ahmed, Rafi .

VACCINE, 2015, 33 :B21-B28

[14]

Lindberg ALE, 2003, VET QUART, V25, P1

[15] New Th2 adjuvants for preventive and active immunotherapy of neurodegenerative proteinopathies [J].

Marciani, Dante J. .

DRUG DISCOVERY TODAY, 2014, 19 (07) :912-920

[16] Current status of veterinary vaccines [J].

Meeusen, Els N. T. ;

Walker, John ;

Peters, Andrew ;

Pastoret, Paul-Pierre ;

Jungersen, Gregers .

CLINICAL MICROBIOLOGY REVIEWS, 2007, 20 (03) :489-510

[17]

Mosmann T R, 1997, Semin Immunol, V9, P87, DOI 10.1006/smim.1997.0065

[18] New generation adjuvants - From empiricism to rational design [J].

O'Hagan, Derek T. ;

Fox, Christopher B. .

VACCINE, 2015, 33 :B14-B20

[19] Key roles of adjuvants in modern vaccines [J].

Reed, Steven G. ;

Orr, Mark T. ;

Fox, Christopher B. .

NATURE MEDICINE, 2013, 19 (12) :1597-1608

[20] Alum-type Adjuvant Effect of Non-haemolytic Saponins Purified from Ilex and Passiflora spp. [J].

Silveira, F. ;

Rossi, S. ;

Fernandez, C. ;

Gosmann, G. ;

Schenkel, E. ;

Ferreira, F. .

PHYTOTHERAPY RESEARCH, 2011, 25 (12) :1783-1788

← 1 2 3 →