IgM, IgG, and IgA Anti-DNA Topoisomerase I Antibodies in Systemic Sclerosis

被引:6
|
作者
Martinez-Cordero, Erasmo [1 ]
Padilla Trejo, Alejandro [1 ]
Aguilar Leon, Diana E. [2 ]
机构
[1] SSA, Res Unit, INER, Natl Inst Resp Dis, Mexico City, DF, Mexico
[2] Natl Inst Med Sci & Nutr Salvador Zubiran, Mexico City, DF, Mexico
关键词
scleroderma; connective tissue disease; autoantibodies; antinuclear antibodies; recombinant protein; immunoglobulins; ANTINUCLEAR ANTIBODIES; ANTICENTROMERE ANTIBODIES; ANTI-SCL-70; ANTIBODIES; SCL-70; AUTOANTIBODIES; SCLERODERMA; SERA; ENZYME; IDENTIFICATION; CENTROMERE;
D O I
10.1002/jcla.20342
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Anti-DNA topoisomerase I (anti-topo I) antibodies have been broadly studied in systemic sclerosis (SSc). The use of different native and molecularly cloned antigens has shown a predominant IgG response, and a variable frequency of positive IgM and IgA tests. We report herein the serological findings of SSc using a recombinant topo I obtained through a standard bacterial system. Methods: Anti-topo I antibodies were studied in 45 SSc patients and 85 healthy controls through ELISA and western blot. Eschenchia coli XL1-blue strain and pT7-7 vector were used to amplify a DNA topo I cDNA clone, and to obtain the recombinant polypeptide. The latter was purified by affinity chromatography, and the enzymatic and antigenic properties were evaluated through specific tests. A native antigen was included for comparison. Results: The SSc group disclosed positive IgM (20%), IgG (86.6%), and IgA (26.6%) anti-topo I tests with the recombinant polypeptide, and a purified calf thymus antigen yielded similar results. IgG autoantibodies were frequently associated with skin involvement, esophageal dysfunction, and restrictive lung disease. The recombinant protein showed a molecular weight of 86.6 kDa, a positive topo I activity using a supercoiled pBR322 DNA relaxation test, and its carboxyl terminus region was recognized by specific antibodies. Conclusion: This report confirms that different immunoglobulin classes with ant-itopo I activity may occur in SSc. IgG was the predominant serological feature with both, the recombinant and native antigens. The study also demonstrates the association between high levels of these autoantibodies and some clinical manifestations of SSc. J. Clin. Lab. Anal. 23:408-416, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:408 / 416
页数:9
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