Quadruplex DNA-Stabilising Dinuclear Platinum(II) Terpyridine Complexes with Flexible Linkers

被引：28

作者：

Ang, Dale L. ^{[1
]}

Harper, Benjamin W. J. ^{[1
]}

Cubo, Leticia ^{[2
]}

Mendoza, Oscar ^{[2
]}

Vilar, Ramon ^{[2
,3
]}

Aldrich-Wright, Janice ^{[1
]}

机构：

[1] Univ Western Sydney, Nanoscale Org & Dynam Grp, Sch Sci & Hlth, Penrith, NSW 2751, Australia

[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[3] Univ London Imperial Coll Sci Technol & Med, Inst Chem Biol, London SW7 2AZ, England

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2016年 / 22卷 / 07期

基金：

英国工程与自然科学研究理事会;

关键词：

circular dichroism; DNA; FRET; G-quadruplexes; platinum; RADIATION CIRCULAR-DICHROISM; TELOMERIC G-QUADRUPLEX; SYNCHROTRON-RADIATION; METAL-COMPLEXES; STRUCTURAL BASIS; SPECTROSCOPY; MYC; STANDARDIZATION; VISUALIZATION; POLYMORPHISM;

D O I：

10.1002/chem.201503663

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Four dinuclear terpyridineplatinum(II) (Pt-terpy) complexes were investigated for interactions with G-quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 degrees C and maintain this in up to a 600-fold excess of dsDNA. This study demonstrates that dinuclear Pt-terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA.

引用

页码：2317 / 2325

页数：9

共 50 条

[1] Solution structure of the biologically relevant g-quadruplex element in the human c-MYC promoter. implications for g-quadruplex stabilization
Ambrus, A
Chen, D
Dai, JX
Jones, RA
Yang, DZ
[J]. BIOCHEMISTRY, 2005, 44 (06) : 2048 - 2058
[2] Targeting G-quadruplexes in gene promoters: a novel anticancer strategy?
Balasubramanian, Shankar
Hurley, Laurence H.
Neidle, Stephen
[J]. NATURE REVIEWS DRUG DISCOVERY, 2011, 10 (04) : 261 - 275
[3] The importance of metal geometry in the recognition of G-quadruplex-DNA by metal-terpyridine complexes
Bertrand, Helene
Monchaud, David
De Cian, Anne
Guillot, Regis
Mergny, Jean-Louis
Teulade-Fichou, Marie-Paule
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (16) : 2555 - 2559
[4] G-Quadruplex Structures in the Human Genome as Novel Therapeutic Targets
Bidzinska, Joanna
Cimino-Reale, Graziella
Zaffaroni, Nadia
Folini, Marco
[J]. MOLECULES, 2013, 18 (10) : 12368 - 12395
[5] Biffi G, 2013, NAT CHEM, V5, P182, DOI [10.1038/nchem.1548, 10.1038/NCHEM.1548]
[6] Böckelman C, 2012, CANCER BIOL THER, V13, P289, DOI [10.4161/cbt.13.5.18922, 10.4161/cbt.18922]
[7] Combining aspects of the platinum anticancer drugs picoplatin and BBR3464 to synthesize a new family of sterically hindered dinuclear complexes; their synthesis, binding kinetics and cytotoxicity
Brown, Sarah D.
Trotter, Katherine D.
Sutcliffe, Oliver B.
Plumb, Jane A.
Waddell, Bruce
Briggs, Naomi E. B.
Wheate, Nial J.
[J]. DALTON TRANSACTIONS, 2012, 41 (37) : 11330 - 11339
[8] Molecular Basis of Structure-Activity Relationships between Salphen Metal Complexes and Human Telomeric DNA Quadruplexes
Campbell, Nancy H.
Abd Karim, Nurul H.
Parkinson, Gary N.
Gunaratnam, Mekala
Petrucci, Vanessa
Todd, Alan K.
Vilar, Ramon
Neidle, Stephen
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 2012, 55 (01) : 209 - 222
[9] Structural Basis for Telomeric G-Quadruplex Targeting by Naphthalene Diimide Ligands
Collie, Gavin W.
Promontorio, Rossella
Hampel, Sonja M.
Micco, Marialuisa
Neidle, Stephen
Parkinson, Gary N.
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (05) : 2723 - 2731
[10] Structure of the intramolecular human telomeric G-quadruplex in potassium solution: a novel adenine triple formation
Dai, Jixun
Punchihewa, Chandanamali
Ambrus, Attila
Chen, Ding
Jones, Roger A.
Yang, Danzhou
[J]. NUCLEIC ACIDS RESEARCH, 2007, 35 (07) : 2440 - 2450

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