Molecular characterization of a cell wall-associated β(1-3)endoglucanase of Aspergillus fumigatus

被引：0
|
作者：
Mouyna, I
Sarfati, J
Recco, P
Fontaine, T
Henrissat, B
Latge, JP
机构：
[1] Inst Pasteur, Unite Aspergillus, F-75724 Paris 15, France
[2] CHU Rangueil, Serv Parasitol Mycol, F-31054 Toulouse, France
[3] CNRS, IFRI, Architecture & Fonct Macromol Biol, Marseille, France
来源：
MEDICAL MYCOLOGY | 2002年 / 40卷 / 05期
关键词：
Aspergillus fumigatus; beta(1-3)endoglucanase; cell wall; ENGL1;
D O I：
10.1080/714031140
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
引用
收藏
页码：455 / 464
页数：10
相关论文
共 50 条
  • [21] Afcwh41 is required for cell wall synthesis, conidiation, and polarity in Aspergillus fumigatus
    Zhang, Lei
    Zhou, Hui
    Ouyang, Haomiao
    Li, Yanjie
    Jin, Cheng
    FEMS MICROBIOLOGY LETTERS, 2008, 289 (02) : 155 - 165
  • [22] Cloning and expression of an intronless gene encoding endoglucanase from Aspergillus fumigatus MKU1
    Meera, B.
    Vanitha, M. C.
    Ramani, G.
    Manjula, A.
    Gunasekaran, P.
    INDIAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (04): : 480 - 486
  • [23] Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus
    Gomez-Lopez, Alicia
    Fernandez-Fernandez, Candela
    BIOFILM, 2024, 8
  • [24] Genetic and structural validation of phosphomannomutase as a cell wall target in Aspergillus fumigatus
    Zhang, Yuanwei
    Fang, Wenxia
    Raimi, Olawale G.
    Lockhart, Deborah E. A.
    Ferenbach, Andrew T.
    Lu, Ling
    van Aalten, Daan M. F.
    MOLECULAR MICROBIOLOGY, 2021, 116 (01) : 245 - 259
  • [25] Comparative Analysis of the Aspergillus fumigatus Cell Wall Modification and Ensuing Human Dendritic Cell Responses by β-(1,3)-Glucan Synthase Inhibitors-Caspofungin and Enfumafungin
    Guilloux, Karine
    Hegde, Pushpa
    Wong, Sarah Sze Wah
    Aimanianda, Vishukumar
    Bayry, Jagadeesh
    Latge, Jean-Paul
    MYCOPATHOLOGIA, 2024, 189 (05)
  • [26] Wall-associated kinase WAK1 interacts with cell wall pectins in a calcium-induced conformation
    Decreux, A
    Messiaen, J
    PLANT AND CELL PHYSIOLOGY, 2005, 46 (02) : 268 - 278
  • [27] The Glycosylphosphatidylinositol-Anchored DFG Family Is Essential for the Insertion of Galactomannan into the β-(1,3)-Glucan-Chitin Core of the Cell Wall of Aspergillus fumigatus
    Muszkieta, Laetitia
    Fontaine, Thierry
    Beau, Remi
    Mouyna, Isabelle
    Vogt, Marian Samuel
    Trow, Jonathan
    Cormack, Brendan P.
    Essen, Lars-Oliver
    Jouvion, Gregory
    Latge, Jean-Paul
    MSPHERE, 2019, 4 (04):
  • [28] ACTIVITY OF CELL WALL-ASSOCIATED ENZYMES IN RIPENING OLIVE FRUIT
    FERNANDEZBOLANOS, J
    RODRIGUEZ, R
    GUILLEN, R
    JIMENEZ, A
    HEREDIA, A
    PHYSIOLOGIA PLANTARUM, 1995, 93 (04) : 651 - 658
  • [29] The Cell Wall-Associated Proteins in the Dimorphic Pathogenic Species of Paracoccidioides
    Puccia, Rosana
    Vallejo, Milene C.
    Longo, Larissa V. G.
    CURRENT PROTEIN & PEPTIDE SCIENCE, 2017, 18 (11) : 1074 - 1089
  • [30] The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis in Aspergillus fumigatus
    Chen, Yuan
    Le Mauff, Francois
    Wang, Yan
    Lu, Ruiyang
    Sheppard, Donald C.
    Lu, Ling
    Zhang, Shizhu
    MBIO, 2020, 11 (06): : 1 - 16
12345