Measurement of Total Antioxidant Capacity by Electrogenerated Iodine at Disposable Screen Printed Electrodes

被引：10

作者：

Isabel Gonzalez-Sanchez, Maria ^{[1
]}

Agrisuelas, Jeronimo ^{[1
]}

Valero, Edelmira ^{[1
]}

Compton, Richard G. ^{[2
]}

机构：

[1] Univ Castilla La Mancha, Dept Phys Chem, Sch Ind Engn, Campus Univ S-N, Albacete 02071, Spain

[2] Univ Oxford, Dept Chem, Phys & Theoret Chem Lab, S Parks Rd, Oxford OX1 3QZ, England

来源：

ELECTROANALYSIS | 2017年 / 29卷 / 05期

关键词：

Disposable screen-printed electrodes; cyclic voltammetry; total antioxidant capacity; ascorbate; iodide; iodine redox couple; plant extracts; ASCORBIC-ACID; AMPEROMETRIC DETECTION; CYCLIC VOLTAMMETRY; INJECTION ANALYSIS; OXIDATIVE STRESS; FLOW-INJECTION; GLUTATHIONE; PLANTS; CHLOROPLASTS; ASSAY;

D O I：

10.1002/elan.201600797

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Total antioxidant capacity is an important parameter for the evaluation of the oxidative status in different kinds of biological samples such as plant extracts, or in food industry. We report a fast, easy, portable, cost-effective electroanalytical method to measure total antioxidant capacity, based on the reaction of natural antioxidants with electrogenerated iodine using disposable platinum screen-printed electrodes. This reaction can be measured by the increment of the electrochemical current signal of iodide oxidation to iodine during a voltammetric cycle. Iodine reacts with reducing compounds such as glutathione, ascorbate, gallic acid and NADH without interference of the corresponding oxidized counter-parts. The addition of ascorbate oxidase also allows the concentration of ascorbate to be determined. The method was tested with real samples of plant extracts and the results correlated well with those obtained with a standard spectrophotometric method.

引用

页码：1316 / 1323

页数：8

共 48 条

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