Double-Coated Poly(butyl Cyanoacrylate) Nanoparticles as a Potential Carrier for Overcoming P-Gp- and BCRP-Mediated Multidrug Resistance in Cancer Cells

被引:4
作者
Kaushal, Neeraj [1 ]
Chen, Zhe-Sheng [1 ]
Lin, Senshang [1 ]
机构
[1] St Johns Univ, Coll Pharm & Hlth Sci, Queens, NY 11432 USA
来源
FRONTIERS IN NANOTECHNOLOGY | 2021年 / 3卷
关键词
doxorubicin; multidrug resistant; nanoparticles; P-glycoprotein (P-gp); transporters; breast cancer resistant protein (BCRP); GLYCOPROTEIN; DOXORUBICIN; INHIBITOR; BRAIN;
D O I
10.3389/fnano.2021.753857
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
The present study evaluates poly (butyl cyanoacrylate) nanoparticles (PBCA-NPs), double-coated with Tween 80 and polyethylene glycol (PEG) 20,000 as a potential carrier system for overcoming P-glycoprotein (P-gp) and breast cancer resistant protein (BCRP)-mediated multidrug resistance (MDR) in cancer cell lines. Doxorubicin-loaded PBCA-NPs were prepared by the anionic polymerization method and were successively double-coated with Tween 80 and PEG 20000 at varied concentrations. MDR reversing potential was investigated by cellular uptake in P-gp overexpressing cell line. And, the outcomes were verified by modified MTT assay in P-gp or BCRP overexpressing cell lines. The findings from the cell uptake study indicate that double-coated PBCA-NPs significantly enhanced doxorubicin accumulation within the cells. MTT assays revealed that double-coated PBCA-NPs significantly potentiated the sensitivity of doxorubicin in P-gp overexpressing cells, in comparison to free doxorubicin, single-, and un-coated PBCA-NPs, respectively. Moreover, further increase in concentration with Tween 80, double-coated PBCA-NPs significantly enhanced the sensitivity of doxorubicin in BCRP overexpressing cell line, in comparison to single- and double-coated formulations (with lower concentration of Tween 80). Hence, it could be concluded that double-coated PBCA-NPs can be used as a potential carrier for enhancing doxorubicin accumulation in MDR cancer cells.
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页数:13
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共 30 条
[1]   Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles [J].
Cabeza, Laura ;
Ortiz, Raul ;
Arias, Jose L. ;
Prados, Jose ;
Ruiz Martinez, Maria Adolfina ;
Entrena, Jose M. ;
Luque, Raquel ;
Melguizo, Consolacion .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2015, 10 :1291-1306
[2]  
Chemical Society American, 2020, CANC FACTS FIG
[3]   Physicochemical evaluation of PLA nanoparticles stabilized by water-soluble MPEO-PLA block copolymers [J].
Chognot, D ;
Six, JL ;
Leonard, M ;
Bonneaux, F ;
Vigneron, C ;
Dellacherie, E .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2003, 268 (02) :441-447
[4]  
Crowley E, 2010, METHODS MOL BIOL, V596, P405, DOI 10.1007/978-1-60761-416-6_18
[5]   Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems [J].
Danaei, M. ;
Dehghankhold, M. ;
Ataei, S. ;
Davarani, F. Hasanzadeh ;
Javanmard, R. ;
Dokhani, A. ;
Khorasani, S. ;
Mozafari, M. R. .
PHARMACEUTICS, 2018, 10 (02)
[6]   Double-coated poly (butylcynanoacrylate) nanoparticulate delivery systems for brain targeting of dalargin via oral administration [J].
Das, D ;
Lin, SS .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2005, 94 (06) :1343-1353
[7]   Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles [J].
Duan, Jinghua ;
Mansour, Heidi M. ;
Zhang, Yangde ;
Deng, Xingming ;
Chen, Yuxiang ;
Wang, Jiwei ;
Pan, Yifeng ;
Zhao, Jinfeng .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2012, 426 (1-2) :193-201
[8]   Radical Polymerization of Alkyl 2-Cyanoacrylates [J].
Duffy, Cormac ;
Zetterlund, Per B. ;
Aldabbagh, Fawaz .
MOLECULES, 2018, 23 (02)
[9]   Microparticles and their emerging role in cancer multidrug resistance [J].
Gong, J. ;
Jaiswal, R. ;
Mathys, J. -M. ;
Combes, V. ;
Grau, G. E. R. ;
Bebawy, M. .
CANCER TREATMENT REVIEWS, 2012, 38 (03) :226-234
[10]   Multidrug resistance in cancer: Role of ATP-dependent transporters [J].
Gottesman, MM ;
Fojo, T ;
Bates, SE .
NATURE REVIEWS CANCER, 2002, 2 (01) :48-58