Isolation, characterization and complete genome sequence of Phaxl: a phage of Escherichia coli O157: H7

被引：32
作者：
Shahrbabak, Salehe Sabouri [1 ,2 ,3 ]
Khodabandehlou, Zahra [1 ,2 ]
Shahverdi, Ahmad Reza [1 ,2 ]
Skurnik, Mikael [4 ,5 ]
Ackermann, Hans-Wolfgang [6 ]
Varjosalo, Markku [7 ]
Yazdi, Mojtaba Tabatabaei [1 ,2 ]
Sepehrizadeh, Zargham [1 ,2 ]
机构：
[1] Univ Tehran Med Sci, Fac Pharm, Dept Pharmaceut Biotechnol, Tehran, Iran
[2] Univ Tehran Med Sci, Fac Pharm, Biotechnol Res Ctr, Tehran, Iran
[3] Kerman Univ Med Sci, Pharmaceut Res Ctr, Kerman, Iran
[4] Univ Helsinki, Dept Bacteriol & Immunol, Haartman Inst, Helsinki, Finland
[5] Univ Helsinki, Cent Hosp Lab, Helsinki, Finland
[6] Univ Laval, Fac Med, Dept Microbiol Immunol & Infectiol, Quebec City, PQ G1X 4C6, Canada
[7] Univ Helsinki, Inst Biotechnol, Helsinki, Finland
来源：
MICROBIOLOGY-SGM | 2013年 / 159卷
关键词：
SALMONELLA-ENTERICA; SEROTYPE O157-H7; BACTERIOPHAGE; IDENTIFICATION; TRANSCRIPTION; BIOCONTROL; PREDICTION; RESISTANCE; RECEPTOR; CATTLE;
D O I：
10.1099/mic.0.063776-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, Phaxl, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115x15 nm. Phaxl contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. Phaxl lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. Phaxl is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.
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收藏
页码：1629 / 1638
页数：10
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