Down-regulation of CRTAC1 attenuates UVB-induced pyroptosis in HLECs through inhibiting ROS production

被引：24
作者：
Sun, Yang [1 ,2 ,3 ,4 ]
Rong, Xianfang [1 ,2 ,3 ,4 ]
Li, Dan [1 ,2 ,3 ,4 ]
Jiang, Yongxiang [1 ,2 ,3 ,4 ]
Lu, Yi [1 ,2 ,3 ,4 ]
Ji, Yinghong [1 ,2 ,3 ,4 ]
机构：
[1] Fudan Univ, Eye & ENT Hosp, Eye Inst, Shanghai 200031, Peoples R China
[2] Fudan Univ, Eye & ENT Hosp, Dept Ophthalmol, Shanghai 200031, Peoples R China
[3] Fudan Univ, Chinese Acad Med Sci, NHC Key Lab Myopia, Key Lab Myopia, Shanghai 200031, Peoples R China
[4] Shanghai Key Lab Visual Impairment & Restorat, Shanghai 200031, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 532卷 / 01期
基金：
中国国家自然科学基金;
关键词：
CRTAC1; Cataract; Pyroptosis; Reactive oxygen species; IRRADIATION INDUCED-APOPTOSIS; VISUAL IMPAIRMENT; RADIATION; MECHANISM; CATARACT;
D O I：
10.1016/j.bbrc.2020.07.028
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pyroptosis has been found to be related to diverse ocular diseases, including cataract. Abnormal CRTAC1 expression has been reported to involve in cataract formation by affecting cell apoptosis. Whether CRTAC1 regulates pyroptosis in the formation progress of cataract is completely unknown. Here, we aimed to investigate the regulatory effects of CRTAC1 on pyroptosis and the potential mechanism in the UVB-induced cell damage model. The results showed that the levels of the established pyroptosis markers (NLRP3, active Caspase-1, pro Caspase-1, GSDMD-N, IL-1 beta and IL-18) were significantly increased in cataract patients. The above pyroptosis markers could be obviously induced by UVB-irradiation in human lens epithelial cells (HLECs), while down-regulation of CRTAC1 significantly reversed the UVB-induced pyroptosis. Up-regulation of CRTAC1 promoted HLECs pyroptosis, while the ROS inhibitor N-acetyl-L-cysteine blocked the effects of CRTAC1 overexpression. In conclusion, our findings further suggested that the prominent role of CRTAC1 in cataract formation. (C) 2020 Elsevier Inc. All rights reserved.
引用
收藏
页码：159 / 165
页数：7
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