Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus

被引：14

作者：

Bittencourt-Cunha, Paula Rego ^{[1
,5
]}

Silva-Cardoso, Livia ^{[1
,5
]}

de Oliveira, Giselle Almeida ^{[1
]}

da Silva, Jose Roberto ^{[2
,5
]}

da Silveira, Alan Barbosa ^{[1
,5
]}

Gabriel Kluck, George Eduardo ^{[1
,5
]}

Souza-Lima, Michele ^{[1
,5
]}

Gondim, Katia Calp ^{[1
,5
]}

Dansa-Petretsky, Marilvia ^{[3
,5
]}

Silva, Carlos Peres ^{[4
]}

Masuda, Hatisaburo ^{[1
,5
]}

Cardoso da Silva Neto, Mario Alberto ^{[1
,5
]}

Atella, Georgia Correa ^{[1
,5
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Bioquim Med, Ctr Ciencias Saude, Rio De Janeiro, RJ, Brazil

[2] Univ Fed Rio de Janeiro, Inst Quim, Dept Bioquim, Macae, RJ, Brazil

[3] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Campos Dos Goytacazes, RJ, Brazil

[4] Univ Fed Santa Catarina, Inst Ciencias Biol, Dept Bioquim, Florianopolis, SC, Brazil

[5] Inst Nacl Ciencia & Tecnol Entomol Mol, Rio De Janeiro, RJ, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2013年 / 108卷 / 04期

关键词：

lipid metabolism; lipoprotein; lipid transport; phospholipids; midgut; TRYPANOSOMA-CRUZI; TRIATOMA-INFESTANS; PLASMA-MEMBRANES; LIPID TRANSPORT; LIPOPHORIN; INSECT; DIGESTION; SURFACE; METABOLISM; ABSORPTION;

D O I：

10.1590/S0074-0276108042013016

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). H-3-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of H-3-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of H-3-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs.

引用

页码：494 / 500

页数：7

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