Detection of p53 gene mutations by single strand conformational polymorphism (SSCP) in human acute myeloid leukemia-derived cell lines

被引：29

作者：

Fleckenstein, DS ^{[1
]}

Uphoff, CC ^{[1
]}

Drexler, HG ^{[1
]}

Quentmeier, H ^{[1
]}

机构：

[1] DSMZ German Collect Microorganism & Cell Cultures, Dept Human & Anim Cell Cultures, D-38124 Braunschweig, Germany

来源：

LEUKEMIA RESEARCH | 2002年 / 26卷 / 02期

关键词：

p53 mutation detection; single strand conformational polymorphism; sequencing analysis;

D O I：

10.1016/S0145-2126(01)00107-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have identified new mutations in the p53 gene in 3/11 growth factor-independent and in 2/8 growth factor-dependent human acute myeloid leukemia (AML)-derived cell lines by single-strand conformational polymorphism (SSCP) and sequencing analysis. MEG-01 had a triplet deletion at codon 304; F-36P, NB-4 and MV4-11 showed point mutations at codon 344. F-36P had a second point mutation at codon 270 and NB-4 additionally at codon 319. M-MOK had a nucleotide substitution at codon 191. The frequency of p53 mutations in the cytokine-independent cell lines was comparable to that in the cytokine-dependent lines. These results suggest that loss of Wild type (wt) p53 is not the decisive event causing tumor cells to proliferate in vitro without externally added growth factors. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：207 / 214

页数：8

共 56 条

[1] Specific P53 mutations are associated with de novo resistance to doxorubicin in breast cancer patients [J].