A new technique for reversible permeabilization of live cells for intracellular delivery of quantum dots

被引:27
作者
Medepalli, Krishnakiran [1 ]
Alphenaar, Bruce W. [1 ]
Keynton, Robert S. [2 ]
Sethu, Palaniappan [2 ]
机构
[1] Univ Louisville, Dept Elect & Comp Engn, Speed Sch Engn, Louisville, KY 40208 USA
[2] Univ Louisville, Dept Bioengn, Speed Sch Engn, Louisville, KY 40208 USA
关键词
CARBON NANOTUBES; MAMMALIAN-CELLS; ORGANIZATION; EFFICIENT; RELEASE;
D O I
10.1088/0957-4484/24/20/205101
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
A major challenge with the use of quantum dots (QDs) for cellular imaging and biomolecular delivery is the attainment of QDs freely dispersed inside the cells. Conventional methods such as endocytosis, lipids based delivery and electroporation are associated with delivery of QDs in vesicles and/or as aggregates that are not monodispersed. In this study, we demonstrate a new technique for reversible permeabilization of cells to enable the introduction of freely dispersed QDs within the cytoplasm. Our approach combines osmosis driven fluid transport into cells achieved by creating a hypotonic environment and reversible permeabilization using low concentrations of cell permeabilization agents like Saponin. Our results confirm that highly efficient endocytosis-free intracellular delivery of QDs can be accomplished using this method. The best results were obtained when the cells were treated with 50 mu g ml(-1) Saponin in a hypotonic buffer at a 3:2 physiological buffer:DI water ratio for 5 min at 4 degrees C.
引用
收藏
页数:13
相关论文
共 35 条
[1]   Applications of carbon nanotubes in drug delivery [J].
Bianco, A ;
Kostarelos, K ;
Prato, M .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2005, 9 (06) :674-679
[2]   Delivering quantum dots to cells: bioconjugated quantum dots for targeted and nonspecific extracellular and intracellular imaging [J].
Biju, Vasudevanpillai ;
Itoh, Tamitake ;
Ishikawa, Mitsuru .
CHEMICAL SOCIETY REVIEWS, 2010, 39 (08) :3031-3056
[3]   Semiconductor nanocrystals as fluorescent biological labels [J].
Bruchez, M ;
Moronne, M ;
Gin, P ;
Weiss, S ;
Alivisatos, AP .
SCIENCE, 1998, 281 (5385) :2013-2016
[4]   Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].
Chan, WCW ;
Nie, SM .
SCIENCE, 1998, 281 (5385) :2016-2018
[5]   Tracking individual kinesin motors in living cells using single quantum-dot imaging [J].
Courty, Sebastien ;
Luccardini, Camilla ;
Bellaiche, Yohanns ;
Cappello, Giovanni ;
Dahan, Maxime .
NANO LETTERS, 2006, 6 (07) :1491-1495
[6]   Intracellular delivery of quantum dots for live cell labeling and organelle tracking [J].
Derfus, AM ;
Chan, WCW ;
Bhatia, SN .
ADVANCED MATERIALS, 2004, 16 (12) :961-+
[7]  
Derfus AM, 2004, NANO LETT, V4, P11, DOI 10.1021/nl0347334
[8]   Intracellular delivery of 2-deoxy-D-glucose into tumor cells by long-term cultivation and through swelling-activated pathways: Implications for radiation treatment [J].
Djuzenova, Cholpon S. ;
Krasnyanska, Julia ;
Kiesel, Martin ;
Stingl, Lavinia ;
Zimmermann, Ulrich ;
Flentje, Michael ;
Sukhorukov, Vladimir L. .
MOLECULAR MEDICINE REPORTS, 2009, 2 (04) :633-640
[9]   In vivo imaging of quantum dots encapsulated in phospholipid micelles [J].
Dubertret, B ;
Skourides, P ;
Norris, DJ ;
Noireaux, V ;
Brivanlou, AH ;
Libchaber, A .
SCIENCE, 2002, 298 (5599) :1759-1762
[10]   Cancer nanotechnology: Opportunities and challenges [J].
Ferrari, M .
NATURE REVIEWS CANCER, 2005, 5 (03) :161-171