Identification of Toxoplasma gondii protein fractions induce immune response against melanoma in mice

被引：11
作者：
Boghozian, Roobina [1 ]
Saei, Azad [1 ]
Mirzaei, Reza [1 ]
Jamali, Arezoo [2 ]
Vaziri, Behrooz [3 ]
Razavi, Alireza [4 ]
Hadjati, Jamshid [1 ]
机构：
[1] Univ Tehran Med Sci, Sch Med, Dept Immunol, Tehran, Iran
[2] Univ Tehran Med Sci, Sch Paramed, Dept Lab Sci, Tehran, Iran
[3] Inst Pasteur, Biotechnol Res Ctr, Tehran, Iran
[4] Univ Tehran Med Sci, Sch Publ Hlth, Dept Immunol, Tehran, Iran
来源：
APMIS | 2015年 / 123卷 / 09期
关键词：
Dendritic cells; immune response; Toxoplasma gondii; tumor; DENDRITIC CELL MATURATION; NECROSIS-FACTOR-ALPHA; LISTERIA-MONOCYTOGENES; CANCER VACCINES; ACTIVATION; DNA; MACROPHAGES; IMMUNOTHERAPY; GENERATION; RECEPTORS;
D O I：
10.1111/apm.12420
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Dendritic cells (DCs) play a crucial role in the initiation of adaptive immune responses against tumor cells. We recently found that protein components of Toxoplasmagondii (T.gondii) could mature DCs efficiently. Therefore, in this study, we aimed to find the most effective protein components of T.gondii which are able to mature DCs and consequently instruct immune responses in tumor-bearing mice. Soluble tachyzoite antigens (STAgs) were fractionated by ammonium sulfate precipitation and subsequently by anion-exchange HPLC. Immature DCs (iDCs) were treated by these protein fractions and were monitored for IL-12p70 and IL-10 production. Moreover, the capacity of mature DCs (mDCs) to induce lymphocyte proliferation was investigated. Ultimately, we analyzed the ability of mDCs in instructing immune responses in tumor-bearing mice. We found that ammonium sulfate fraction one (A1) matured-DCs produced higher IL-12 level and IL-12/IL-10 ratio; therefore, this fraction was selected for further fractionation by anion-exchange HPLC. The results showed that anion-exchange HPLC fraction 14 (C14) matured-DCs secrete higher levels of IL-12p70 and IL-12p70/IL-10 ratio. Survival of the mice matured by A1 fraction increased significantly compared to other groups. Moreover, SDS-PAGE electrophoresis showed that different obtained fractions have distinct proteins based on their size. These results demonstrate that two protein fractions of T.gondii are able to mature DCs more efficient.
引用
收藏
页码：800 / 809
页数：10
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