RNA-sequencing Profiles of Cell Cycle-Related Genes Upregulated during the G2-Phase in Giardia lamblia

被引：9
作者：
Kim, Juri [1 ,2 ]
Shin, Mee Young [1 ,2 ]
Park, Soon-Jung [1 ,2 ]
机构：
[1] Yonsei Univ, Coll Med, Dept Environm Med Biol, Seoul 03722, South Korea
[2] Yonsei Univ, Coll Med, Inst Trop Med, Seoul 03722, South Korea
来源：
KOREAN JOURNAL OF PARASITOLOGY | 2019年 / 57卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Giardia lamblia; RNA-sequence; cell cycle; G2-phase;
D O I：
10.3347/kjp.2019.57.2.185
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.
引用
收藏
页码：185 / +
页数：6
相关论文
共 39 条
  • [31] Cell Cycle Arrest in G2/M Phase Enhances Replication of Interferon-Sensitive Cytoplasmic RNA Viruses via Inhibition of Antiviral Gene Expression
    Bressy, Christian
    Droby, Gaith N.
    Maldonado, Bryant D.
    Steuerwald, Nury
    Grdzelishvili, Valery Z.
    [J]. JOURNAL OF VIROLOGY, 2019, 93 (04)
  • [32] Live imaging of the Dictyostelium cell cycle reveals widespread S phase during development, a G2 bias in spore differentiation and a premitotic checkpoint
    Muramoto, Tetsuya
    Chubb, Jonathan R.
    [J]. DEVELOPMENT, 2008, 135 (09): : 1647 - 1657
  • [33] Nuclear phosphoinositide 3-kinase C2β activation during G2/M phase of the cell cycle in HL-60 cells
    Visnjic, D
    Curic, J
    Crljen, V
    Batinic, D
    Volinia, S
    Banfic, H
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2003, 1631 (01): : 61 - 71
  • [34] THE ORIGIN AND ORGANIZATION OF CORTICAL MICROTUBULES DURING THE TRANSITION BETWEEN M-PHASE AND G(1)-PHASE OF THE CELL-CYCLE AS OBSERVED IN HIGHLY SYNCHRONIZED CELLS OF TOBACCO BY-2
    NAGATA, T
    KUMAGAI, F
    HASEZAWA, S
    [J]. PLANTA, 1994, 193 (04) : 567 - 572
  • [35] REGULATION OF EXPRESSION OF THE GROWTH-STATE-RELATED GENES 2F1 AND 2A9 DURING ENTRY OF QUIESCENT SMOOTH-MUSCLE CELLS INTO THE CELL-CYCLE
    KINDY, MS
    BROWN, KE
    SONENSHEIN, GE
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1991, 46 (04) : 345 - 350
  • [36] Tyrosine-phosphorylated extracellular signal-regulated kinase associates with the Golgi complex during G2/M phase of the cell cycle: Evidence for regulation of Golgi structure
    Cha, HJ
    Shapiro, P
    [J]. JOURNAL OF CELL BIOLOGY, 2001, 153 (07) : 1355 - 1367
  • [37] Persistent nuclear accumulation of protein kinase CK2 during the G1-phase of the cell cycle does not depend on the ERK1/2 pathway but requires active protein synthesis
    Miro, FA
    Llorens, F
    Roher, N
    Rana, M
    Gómez, N
    Itarte, E
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2002, 406 (02) : 165 - 172
  • [38] Phosphorylation of CDC25C by AMP-activated protein kinase mediates a metabolic checkpoint during cell-cycle G2/M-phase transition
    Shen, Yuqing
    Sherman, John William
    Chen, Xuyong
    Wang, Ruoning
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2018, 293 (14) : 5185 - 5199
  • [39] Vitamins K2, K3 and K5 exert in vivo antitumor effects on hepatocellular carcinoma by regulating the expression of G1 phase-related cell cycle molecules
    Kuriyama, S
    Hitomi, M
    Yoshiji, H
    Nonomura, T
    Tsujimoto, T
    Mitoro, A
    Akahane, T
    Ogawa, M
    Nakai, S
    Deguchi, A
    Masaki, T
    Uchida, N
    [J]. INTERNATIONAL JOURNAL OF ONCOLOGY, 2005, 27 (02) : 505 - 511
1234