RNA-sequencing Profiles of Cell Cycle-Related Genes Upregulated during the G2-Phase in Giardia lamblia

被引：9
作者：
Kim, Juri [1 ,2 ]
Shin, Mee Young [1 ,2 ]
Park, Soon-Jung [1 ,2 ]
机构：
[1] Yonsei Univ, Coll Med, Dept Environm Med Biol, Seoul 03722, South Korea
[2] Yonsei Univ, Coll Med, Inst Trop Med, Seoul 03722, South Korea
来源：
KOREAN JOURNAL OF PARASITOLOGY | 2019年 / 57卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Giardia lamblia; RNA-sequence; cell cycle; G2-phase;
D O I：
10.3347/kjp.2019.57.2.185
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.
引用
收藏
页码：185 / +
页数：6
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