Alginate/CaCO3 Hybrid Nanoparticles for Efficient Codelivery of Antitumor Gene and Drug

被引：77
作者：
Zhao, Dong [1 ]
Liu, Chuan-Jun [1 ]
Zhuo, Ren-Xi [1 ]
Cheng, Si-Xue [1 ]
机构：
[1] Wuhan Univ, Dept Chem, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Peoples R China
来源：
MOLECULAR PHARMACEUTICS | 2012年 / 9卷 / 10期
基金：
中国国家自然科学基金;
关键词：
drug delivery; gene transfer; calcium carbonate; alginate; nanoparticles; NANOSTRUCTURED CALCIUM-CARBONATE; MESOPOROUS SILICA NANOPARTICLES; MULTIDRUG-RESISTANT CANCER; CO-DELIVERY; ANTICANCER DRUG; MESSENGER-RNA; P53; FUNCTION; DOXORUBICIN; CELLS; SIRNA;
D O I：
10.1021/mp3002123
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
In this study, a facile strategy for efficient codelivery of gene and drug was developed. Using a coprecipitation method, doxorubicin hydrochloride (DOX), an antitumor drug, and p53 expression plasmid were encapsulated in alginate/CaCO3/DNA/DOX nanoparticles with high encapsulation efficiency. The in vitro cell inhibition effect of the alginate/CaCO3/DNA/DOX nanoparticles was evaluated by MTT assay in HeLa cells. The alginate/CaCO3/DNA/DOX nanoparticles exhibited a high cell inhibition rate about 80%, indicating that the alginate/CaCO3/DNA/DOX nanoparticles could effectively mediate gene transfection and deliver the drug to the cells. Compared with the codelivery of gene and drug, the treatments by alginate/CaCO3/DOX nanoparticles and alginate/CaCO3/DNA nanoparticles separately led to much lower cell inhibition rates. Compared with the CaCO3/DNA/DOX nanoparticles without alginate modification, the alginate/CaCO3/DNA/DOX nanoparticles with a decreased particle size exhibited enhanced delivery efficiency. The alginate/CaCO3/DNA/DOX nanoparticles have promising applications in cancer treatments.
引用
收藏
页码：2887 / 2893
页数：7
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