Compound Chinese herbal medicinal ingredients can enhance immune response and efficacy of RHD vaccine in rabbit

被引：68

作者：

Yang, Longsheng ^{[1
,2
]}

Hu, Yuanliang ^{[1
]}

Xue, Jiabin ^{[2
]}

Wang, Fang ^{[2
]}

Wang, Deyun ^{[1
]}

Kong, Xiangfeng ^{[1
,4
,5
]}

Li, Peng ^{[3
]}

Xu, Weizhong ^{[2
]}

机构：

[1] Nanjing Agr Univ, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China

[3] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA

[4] Chinese Acad Sci, Lab Anim Nutr & Human Hlth, Changsha 410125, Hunan, Peoples R China

[5] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol, Changsha 410125, Hunan, Peoples R China

来源：

VACCINE | 2008年 / 26卷 / 35期

基金：

中国国家自然科学基金;

关键词：

compound Chinese herbal medicinal ingredients; rabbit hemorrhagic disease; lymphocyte proliferation; gene expression; antibody titer;

D O I：

10.1016/j.vaccine.2008.06.075

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to validate the immune-enhancement efficacy of compound Chinese herbal medicinal ingredients (cCHMIs), made with astragalus polysaccharide (APS), epimedium polysaccharide (EPS), propolis flavone (PF) and ginsenosides (GS), as immune potentiator or vaccine adjuvants for rabbits, the effects of two cCHMIs on rabbit lymphocyte proliferation and IFN-gamma and IL-10 mRNA expression of T lymphocyte in vitro were determined. At the same time, two cCHMIs were injected into 35-day-old rabbits after mixed with rabbit hemorrhagic disease (RHD) vaccine taking aluminum adjuvant and phosphate-buffered saline (PBS) as controls. On days 7, 14, 21,35 and 49 after the vaccination, the dynamic changes of peripheral lymphocyte proliferation and serum antibody titers of the rabbits were analyzed. On day 63, all rabbits were challenged with RHD virus. The results showed that the two cCHMIs could significantly promote rabbit lymphocyte proliferation and IFN-gamma and IL-10 mRNA expression of T lymphocyte in vitro. In vivo, two cCHMIs could significantly enhance serum antibody titers and lymphocyte proliferation. Their adjuvanticity was slightly superior to aluminum adjuvant. All the rabbits vaccinated with the cCHMIs adjuvant vaccine were protected. These findings confirmed that two cCHMIs possessed better immune-enhancement efficacy and would be used as effective immune adjuvant of RHD vaccine. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：4451 / 4455

页数：5

共 24 条

[1]

ABUL KA, 1997, CELLULAR MOL IMMUNOL, P194

[2]

CANDIDA F, 1998, CLIN IMMUNOL IMMUNOP, V1, P34

[3]

CHU YF, 2004, J NANJING AGR U, V1, P97

[4]

CLARK EA, 1991, ANNU REV IMMUNOL, V9, P97

[5]

Deng XH, 2006, J CHONGQING TECHNOL, V23, P556

[6]

Harvey D. P., 2000, CYTOKINE, V6, P555

[7]

Hu Y, 1997, CHIN J IMMUNOL, V3, P96

[8]

KATIE B, 2004, SEMIN AVIAN EXOT PET, V13, P100

[9] Chinese herbal ingredients are effective immune stimulators for chickens infected with the Newcastle disease virus [J].

Kong, X. -F. ;

Hu, Y. -L. ;

Yin, Y. -L. ;

Wu, G. -Y. ;

Rui, R. ;

Wang, D. -Y. ;

Yang, C. -B. .

POULTRY SCIENCE, 2006, 85 (12) :2169-2175

[10] Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken [J].

Kong, XF ;

Hu, YL ;

Rui, R ;

Wang, DY ;

Li, XG .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2004, 4 (07) :975-982

← 1 2 3 →