Enhanced co-production of hydrogen and poly-(R)-3-hydroxybutyrate by recombinant PHB producing E. coli over-expressing hydrogenase 3 and acetyl-CoA synthetase

被引：30

作者：

Wang, Rui-Yan ^{[1
]}

Shi, Zhen-Yu ^{[2
]}

Chen, Jin-Chun ^{[1
]}

Wu, Qiong ^{[1
]}

Chen, Guo-Qiang ^{[1
,3
]}

机构：

[1] Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, MOE Key Lab Bioinformat & Syst Biol,Dept Biol Sci, Beijing 100084, Peoples R China

[2] Www Synthenome Com, Dingley Village, Vic 3172, Australia

[3] Tsinghua Univ, Ctr Nano & Micro Mech, Beijing 100084, Peoples R China

来源：

METABOLIC ENGINEERING | 2012年 / 14卷 / 05期

基金：

中国国家自然科学基金;

关键词：

PHB; Hydrogen production; Hydrogenase; 3; Synthetic biology; Metabolic engineering; Escherichia coli; ESCHERICHIA-COLI; FORMATE DEHYDROGENASE; LYASE; METABOLISM; ACID; POLY(3-HYDROXYPROPIONATE-CO-4-HYDROXYBUTYRATE); POLY(3-HYDROXYBUTYRATE); POLYHYDROXYALKANOATES; FERMENTATION; COPOLYESTERS;

D O I：

10.1016/j.ymben.2012.07.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant Escherichia coli was constructed for co-production of hydrogen and polyhydroxybutyrate (PHB) due to its rapid growth and convenience of genetic manipulation. In particular, anaerobic metabolic pathways dedicated to co-production of hydrogen and PHB were established due to the advantages of directing fluxes away from toxic compounds such as formate and acetate to useful products. Here, recombinant E. coli expressing hydrogenase 3 and/or acetyl-CoA synthetase showed improved PHB and hydrogen production when grown with or without acetate as a carbon source. When hydrogenase 3 was over-expressed, hydrogen yield was increased from 14 to 153 mmol H-2/mol glucose in a mineral salt (MS) medium with glucose as carbon source, accompanied by an increased PHB yield from 0.55 to 5.34 mg PHB/g glucose in MS medium with glucose and acetate as carbon source. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：496 / 503

页数：8