Puerarin antagonizes peroxyntrite-induced injury in retinal pigment epithelial cells

被引：2
作者：
Hao, Lina [1 ]
Zhang, Xudong [2 ]
Yang, Tao [3 ]
Ma, Junling [1 ]
机构：
[1] Hebei Prov Peoples Hosp, Dept Ophthalmol, Shijiazhuang 050051, Hebei Province, Peoples R China
[2] Hebei Prov Peoples Hosp, Dept Pharmaceut, Shijiazhuang 050051, Hebei Province, Peoples R China
[3] Hebei Med Univ, Hosp 1, Internal Dept, Shijiazhuang 050031, Hebei Province, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2012年 / 7卷 / 09期
基金：
中国国家自然科学基金;
关键词：
apoptosis; Fas/FasL; inducible nitric oxide synthase; oxidative stress; puerarin; retinal pigment epithelial cells; NITRIC-OXIDE SYNTHASE; DIABETIC-RATS; PEROXYNITRITE; EXPRESSION; APOPTOSIS; OXIDATION; STIMULI; PATHWAY; SHEETS;
D O I：
10.3969/j.issn.1673-5374.2012.09.005
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A rat model of diabetes mellitus was established by intraperitoneal injection of streptozotocin. Three days later, the rats were intraperitoneally administered 140 mg puerarin/kg daily, for a total of 60 successive days. DNA ladder results showed increased apoptosis over time in retinal pigment epithelial cells from rats with streptozotocin-induced diabetes mellitus. Western blot analysis, Reverse transcription-PCR, immunohistochemistry, and flow cytometry results showed increased expression of 3-nitrotyrosine, a peroxyntrite marker, as well as inducible nitric synthase and Fas/FasL, in retinal pigment epithelial cells. Puerarin reversed these changes, and results demonstrated that puerarin inhibited Fas/FasL expression and alleviated peroxyntrite injury to retinal pigment epithelial cells. These results suggested that puerarin inhibited production of inducible nitric oxide synthase and directly antagonized peroxyntrite injury in retinal pigment epithelial cells.
引用
收藏
页码：669 / 674
页数：6
相关论文
共 29 条
[1]   Early Intervention of Tyrosine Nitration Prevents Vaso-Obliteration and Neovascularization in Ischemic Retinopathy [J].
Abdelsaid, Mohammed A. ;
Pillai, Bindu A. ;
Matragoon, Suraporn ;
Prakash, Roshini ;
Al-Shabrawey, Mohamed ;
El-Remessy, Azza B. .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2010, 332 (01) :125-134
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   Protective Effects of a Coumarin Derivative in Diabetic Rats [J].
Bucolo, Claudio ;
Ward, Keith W. ;
Mazzon, Emanuela ;
Cuzzocrea, Salvatore ;
Drago, Filippo .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2009, 50 (08) :3846-3852
[4]   Survival of allogeneic porcine retinal pigment epithelial sheets after subretinal transplantation [J].
Del Priore, LV ;
Tezel, TH ;
Kaplan, HJ .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 (03) :985-992
[5]   Enhanced HtrA2/Omi Expression in Oxidative Injury to Retinal Pigment Epithelial Cells and Murine Models of Neurodegeneration [J].
Ding, Xiaoyan ;
Patel, Mrinali ;
Shen, Defen ;
Herzlich, Alexandra A. ;
Cao, Xiaoguang ;
Villasmil, Rafael ;
Klupsch, Kristina ;
Tuo, Jingsheng ;
Downward, Julian ;
Chan, Chi-Chao .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2009, 50 (10) :4957-4966
[6]   Comparative effects of fatty acids on proinflammatory gene cyclooxygenase 2 and inducible nitric oxide synthase expression in retinal pigment epithelial cells [J].
Fang, I-Mo ;
Yang, Chang-Hao ;
Yang, Chung-May ;
Chen, Muh-Shy .
MOLECULAR NUTRITION & FOOD RESEARCH, 2009, 53 (06) :739-750
[7]   Different death stimuli evoke apoptosis via multiple pathways in retinal pigment epithelial cells [J].
Ferrington, Deborah A. ;
Tran, Tina N. ;
Lew, Kathleen L. ;
Remmen, Holly Van ;
Gregerson, Dale S. .
EXPERIMENTAL EYE RESEARCH, 2006, 83 (03) :638-650
[8]   Determination of puerarin by capillary electrophoresis with chemiluminescence detection [J].
Han, Suqin .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (14-15) :1591-1594
[9]  
Hao Li-Na, 2006, Shengli Xuebao, V58, P584
[10]  
Hao Li-Na, 2008, Zhonghua Yan Ke Za Zhi, V44, P163
123