Biosynthesis of benzoylformic acid from benzoyl cyanide by a newly isolated Rhodococcus sp CCZU10-1 in toluene-water biphasic system

被引：29

作者：

He, Yu-Cai ^{[1
,2
]}

Zhou, Qiong ^{[1
]}

Ma, Cui-Luan ^{[1
]}

Cai, Zhi-Qiang ^{[1
]}

Wang, Li-Qun ^{[1
]}

Zhao, Xi-Yue ^{[1
]}

Chen, Qun ^{[1
]}

Gao, Da-Zhou ^{[1
]}

Zheng, Ming ^{[1
]}

Wang, Xu-Dong ^{[1
]}

Sun, Quan ^{[1
]}

机构：

[1] Changzhou Univ, Biochem Engn Lab, Coll Pharmaceut & Life Sci, Changzhou 213164, Peoples R China

[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 115卷

基金：

中国国家自然科学基金;

关键词：

Benzoylformic acid; Benzoyl cyanide; Rhodococcus sp CCZU10-1; Biotransformation; Optimization; ALCALIGENES SP ECU0401; (R)-(-)-MANDELIC ACID; NITRILE HYDROLYSIS; ENANTIOSELECTIVE HYDROLYSIS; RACEMIC MANDELONITRILE; BACTERIAL STRAIN; RHODOCHROUS J1; FAECALIS JM3; WHOLE CELLS; SP AJ270;

D O I：

10.1016/j.biortech.2011.09.084

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Benzoylformic acid was synthesized from the hydrolysis of benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1. In this study, an aqueous-toluene biphasic system was developed for highly efficient production of benzoylformic acid from the hydrolysis of benzoyl cyanide. In the aqueous-toluene biphasic system, the phase volume ratio, buffer pH and reaction temperature were optimized. Using fed-batch method, a total of 932 mM benzoylformic acid accumulated in the reaction mixture after the 10th feed. Moreover, enzymatic hydrolysis of benzoyl cyanide using calcium alginate entrapped resting cells was carried out in the aqueous-toluene biphasic system, and efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 14.26g benzoylformic acid g(-1) dry cell weight (DCW) cell after 20 cycles of repeated use. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：88 / 95

页数：8

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