Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

被引：3

作者：

Fedotov, I. V. ^{[1
,2
,3
]}

Doronina-Amitonova, L. V. ^{[1
,2
,3
]}

Sidorov-Biryukov, D. A. ^{[1
,2
]}

Fedotov, A. B. ^{[1
,2
]}

Anokhin, K. V. ^{[3
,4
]}

Kilin, S. Ya. ^{[5
]}

Sakoda, K. ^{[6
]}

Zheltikov, A. M. ^{[1
,2
,7
,8
]}

机构：

[1] Moscow MV Lomonosov State Univ, Ctr Int Laser, Dept Phys, Moscow 119992, Russia

[2] Russian Quantum Ctr, Skolkovo 1430125, Moscow Region, Russia

[3] Kurchatov Inst Natl Res Ctr, Moscow, Russia

[4] Russian Acad Med Sci, PK Anokhin Inst Normal Physiol, Moscow, Russia

[5] Natl Acad Sci Belarus, BI Stepanov Inst Phys, Minsk, BELARUS

[6] Natl Inst Mat Sci, Tsukuba, Ibaraki 3050044, Japan

[7] Texas A&M Univ, Dept Phys & Astron, College Stn, TX 77843 USA

[8] Russian Acad Sci, Ctr Photochem, Moscow 117421, Russia

来源：

APPLIED PHYSICS LETTERS | 2014年 / 104卷 / 08期

基金：

俄罗斯基础研究基金会;

关键词：

2-PHOTON MICROSCOPE; INTERFACE;

D O I：

10.1063/1.4864646

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen-vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser-fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection. (C) 2014 AIP Publishing LLC.

引用

页数：4

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