A label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood

被引：48

作者：

Sha, Yuhong ^{[1
]}

Zhang, Xuan ^{[2
]}

Li, Wenrou ^{[1
]}

Wu, Wei ^{[3
]}

Wang, Sui ^{[1
]}

Guo, Zhiyong ^{[1
]}

Zhou, Jun ^{[2
]}

Su, Xiurong ^{[2
]}

机构：

[1] Ningbo Univ, Sch Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China

[3] Ningbo Univ, Fac Elect Engn & Comp Sci, Ningbo 315211, Zhejiang, Peoples R China

来源：

TALANTA | 2016年 / 147卷

基金：

中国国家自然科学基金;

关键词：

Electrochemiluminescence immunosensor; Vibrio parahaemolyticus; Multi-functionalized graphene oxide; One-step; MEDIATED ISOTHERMAL AMPLIFICATION; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; ELECTROCHEMICAL IMMUNOSENSOR; LISTERIA-MONOCYTOGENES; NANOPARTICLES; VULNIFICUS; SEPARATION; SHELLFISH; EFFICIENT;

D O I：

10.1016/j.talanta.2015.09.058

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A label-free electrochemiluminescence (ECL) immunosensor for ultrasensitive and rapid detection of marine pathogenic bacterium Vibrio parahaemolyticus (VP) in seawater and seafood was developed based on multi-functionalized graphene oxide, which was prepared with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and VP antibody (anti-VP) simultaneously immobilized on the surface of magnetic graphene oxide (nanoFe(3)O(4)@GO). ABEI and anti-VP acted as the electrochemiluminophore and the capture device for VP respectively. Good conductivity and two-dimensional structure of the nanoFe(3)O(4)@GO enabled all the ABEI, immobilized on GO, electrochemically active and thus improved the detection sensitivity. Under optimal conditions, the ECL intensity decreased with increasing logarithmic concentrations of VP in the range of 10-10(8) CFU/mL, with a detection limit of 5 CFU/mL for seawater and 5 CFU/g for seafood. This ECL immunosensor showed high specificity, stability and reproducibility for the detection of VP. In addition, the ECL immunosensor has been successfully used to determine the concentration of VP in seawater and seafood rapidly, with a recovery of 94.4-112.0% and RSD 4.1-11.7%. Therefore, the developed immunosensor shows great prospect for practical application. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：220 / 225

页数：6

共 45 条

[1] A review of the current status of cultural and rapid detection of Vibrio parahaemolyticus [J].

Bisha, Bledar ;

Simonson, Janet ;

Janes, Marlene ;

Bauman, Kellie ;

Goodridge, Lawrence D. .

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2012, 47 (05) :885-899

[2] Scanometric nanomolar lead (II) detection using DNA-functionalized gold nanoparticles and silver stain enhancement [J].

Chen, Beibei ;

Wang, Zebo ;

Hu, Dongxue ;

Ma, Qingqing ;

Huang, Linna ;

Xv, Chengyin ;

Guo, Zhiyong ;

Jiang, Xiaohua .

SENSORS AND ACTUATORS B-CHEMICAL, 2014, 200 :310-316

[3] Rapid detection of Vibrio parahaemolyticus in oysters by immunofluorescence microscopy [J].

Chen, HC ;

Chang, TC .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1996, 29 (2-3) :311-319

[4] An ultrasensitive sandwich type electrochemiluminescence immunosensor for triiodothyronine detection using silver nanoparticle-decorated graphene oxide as a nanocarrier [J].

Chou, Hung-Tao ;

Fu, Chien-Yu ;

Lee, Chi-Young ;

Tai, Nyan-Hwa ;

Chang, Hwan-You .

BIOSENSORS & BIOELECTRONICS, 2015, 71 :476-482

[5] An overview of Vibrio vulnificus and Vibrio parahaemolyticus [J].

Drake, Stephenie L. ;

DePaola, Angelo ;

Jaykus, Lee-Ann .

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2007, 6 (04) :120-144

[6] Electrochemiluminescence immunosensor for tumor markers based on biological barcode mode with conductive nanospheres [J].

Du, Shuping ;

Guo, Zhiyong ;

Chen, Beibei ;

Sha, Yuhong ;

Jiang, Xiaohua ;

Li, Xing ;

Gan, Ning ;

Wang, Sui .

BIOSENSORS & BIOELECTRONICS, 2014, 53 :135-141

[7] An enhanced sensitive electrochemical immunosensor based on efficient encapsulation of enzyme in silica matrix for the detection of human immunodeficiency virus p24 [J].

Fang, Yi-Shan ;

Huang, Xin-Jian ;

Wang, Li-Shi ;

Wang, Ju-Fang .

BIOSENSORS & BIOELECTRONICS, 2015, 64 :324-332

[8] Electrochemical biosensors on platforms of graphene [J].

Fang, Youxing ;

Wang, Erkang .

CHEMICAL COMMUNICATIONS, 2013, 49 (83) :9526-9539

[9]

FUJINO T., 1953, MED JOUR OSAKA UNIV, V4, P299

[10] Electrochemiluminescence immunosensor based on graphene-CdS quantum dots-agarose composite for the ultrasensitive detection of alpha fetoprotein [J].

Guo, Zhiyong ;

Hao, Tingting ;

Duan, Jing ;

Wang, Sui ;

Wei, Danyi .

TALANTA, 2012, 89 :27-32

← 1 2 3 4 5 →