Amplified detection of DNA by an analyte-induced Y-shaped junction probe assembly followed with a nicking endonuclease-mediated autocatalytic recycling process

被引：19

作者：

Liu, Shufeng ^{[1
]}

Zhang, Chengxin ^{[2
]}

Ming, Jingjing ^{[2
]}

Wang, Chunfeng ^{[2
]}

Liu, Tao ^{[2
]}

Li, Feng ^{[1
]}

机构：

[1] Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China

[2] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 72期

基金：

中国国家自然科学基金;

关键词：

SEQUENCE-SPECIFIC DETECTION; SIGNAL AMPLIFICATION; NUCLEIC-ACIDS; ISOTHERMAL AMPLIFICATION; ULTRASENSITIVE DETECTION; MOLECULAR BEACONS; LABEL-FREE; MICRORNAS; HYBRIDIZATION; MACHINE;

D O I：

10.1039/c3cc45211e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple, isothermal and autocatalytic system for the amplified detection of DNA was ingeniously designed based on target-triggered Y-shaped junction probe assembly, followed with a nicking endonuclease-mediated cascade recycling process. A superior detection limit of 0.1 pM towards the target DNA with an excellent selectivity could be achieved.

引用

页码：7947 / 7949

页数：3

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