Sambucus Williamsii Hance Promotes MC3T3-E1 Cells Proliferation and Differentiation via BMP-2/Smad/p38/JNK/Runx2 Signaling Pathway

被引:25
作者
Yang, Bingyou [1 ]
Lin, Xiaoying [1 ]
Yang, Chunli [1 ]
Tan, Jinyan [1 ]
Li, Wei [2 ]
Kuang, Haixue [1 ]
机构
[1] Heilongjiang Univ Chinese Med, Minist Educ, Key Lab Chinese Mat Med, Harbin 150040, Peoples R China
[2] Heilongjiang Univ Chinese Med, Affiliated Hosp 1, Natl Key Lab Gynecol, Dept Obstet & Gynecol, Harbin 150040, Peoples R China
基金
中国国家自然科学基金;
关键词
Sambucus Williamsii Hance; bone fractures; MC3T3-E1; BMP-2; MAPK; Runx2; ACTIVATED PROTEIN-KINASE; BONE MORPHOGENETIC PROTEINS; OSTEOBLAST DIFFERENTIATION; EXPRESSION; INDUCTION; CASCADES; LIGNANS;
D O I
10.1002/ptr.5482
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The 50% ethanol elution fractions of root-bark of Sambucus Williamsii Hance (rbSWH) evaluated the effect of proliferation and differentiation on preosteoblast MC3T3-E1 cell, and the mechanism of actions. We found that rbSWH(30, 60, and 90 mu g/mL) can enhance cell proliferation by MTT assay and promote alkaline phosphatase (ALP) and bone Gla protein (BGP) activities, type I collagen (Col-I) synthesis, and mineralization nodule formation in primary cultured osteoblasts. The results showed that rbSWH can increase mRNA levels of BMP-2 and Runx2 using real-time reverse transcription-quantitative polymerase chain reaction, whereas the BMP-2 antagonist Noggin attenuated the increase of ALP activity induced by rbSWH, indicating that BMP-2 expression was required for the action of rbSWH in osteoblastic. We also found that rbSWH can enhance the expressions of BMP-2, BMPRIB, BMPRII, phosphorylation of Smad, JNK and p38, and Runx2 proteins by western blotting. In addition, pretreatment of cells with p38 inhibitor (SB203580) or JNK inhibitor (SP600125) can antagonize the elevation of BMP-2 expression, ALP activity, and cell viability induced by rbSWH. Taken together, our results provided an evidence that rbSWH can promote MC3T3-E1 cell proliferation and differentiation via BMP-2/Smad/p38/JNK/Runx2 signaling pathway. Copyright (C) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码:1692 / 1699
页数:8
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