Quantitative real-time PCR for detection of the neurotoxin gene of Clostridium botulinum type B in equine and bovine samples

被引：13

作者：

Johnson, Amy L. ^{[1
]}

Sweeney, Raymond W. ^{[1
]}

McAdams, Susan C. ^{[1
]}

Whitlock, Robert H. ^{[1
]}

机构：

[1] Univ Penn, Sch Vet Med, New Bolton Ctr, Dept Clin Studies, Kennett Sq, PA 19348 USA

来源：

VETERINARY JOURNAL | 2012年 / 194卷 / 01期

关键词：

Botulism; Equine; Bovine; Diagnosis; PCR; Mouse bioassay; CATTLE; TOXIN;

D O I：

10.1016/j.tvjl.2012.03.018

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Clostridium botulinum type B is estimated to cause more than 85% of cases of equine botulism in the united States, as well as many outbreaks in cattle. In this study, a quantitative real-time polymerase chain reaction for detection of the neurotoxin gene of C botulinum type B was compared to the mouse bioassay using 45 positive and 43 negative samples of equine, bovine or associated environmental origin. The sensitivity of the qPCR assay was 96%, whereas the sensitivity of the mouse bioassay was 84%. The specificity of the qPCR assay was 95% and the specificity of the mouse bioassay was 100%. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：118 / 120

页数：3

共 10 条

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