In vitro and in vivo protective effect of honokiol on rat liver from peroxidative injury

被引:64
作者
Chiu, JH
Ho, CT
Wei, YH
Lui, WY
Hong, CY
机构
[1] VET GEN HOSP,DEPT SURG,DIV GEN SURG,TAIPEI 11217,TAIWAN
[2] NATL YANG MING UNIV,DEPT BIOCHEM,TAIPEI 112,TAIWAN
关键词
honokiol; lipid peroxidation; reperfusion injury;
D O I
10.1016/S0024-3205(97)00836-9
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Honokiol, a compound extracted from the Chinese medicinal herb Magnolia officinalis, has a strong antioxidant effect on the inhibition of lipid peroxidation in rat heart mitochondria. To investigate the protective effect of honokiol on hepatocytes from peroxidative injury, oxygen consumption and malondialdehyde formation for in vitro iron-induced lipid peroxidation were assayed, and the mitochondrial respiratory function for in vivo ischemia-reperfusion injury were evaluated in rat liver, respectively. The inhibitory effect of honokiol on oxygen consumption and malondialdehyde formation during iron-induced lipid peroxidation in liver mitochondria showed obvious dose-dependent responses with a concentration of 50% inhibition being 2.3x10(-7)M and 4.96x10(-7)M, respectively, that is, 550 times and 680 rimes more potent than alpha-tocopherol, respectively. When rat livers were introduced with ischemia 60 min followed by reperfusion for 60 min, and then pretreated with honokiol (10 ug/kg BW), the mitochondrial respiratory control ratio (the quotient of the respiration rate of State 3 to that of State 4) and ADP/O ratio from the honokiol-treated livers were significantly higher than those of non-treated livers during reperfusion. The dose-dependent protective effect of honokiol on ischemia-reperfusion injury was 10 mu g - 100 mu g/Kg body weight. We conclude that honokiol is a strong antioxidant and shed insight into clinical implications for protection of hepatocytes from ischemia-reperfusion injury.
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页码:1961 / 1971
页数:11
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