Acid trehalase is involved in intracellular trehalose mobilization during postdiauxic growth and severe saline stress in Saccharomyces cerevisiae

被引：19
作者：
Garre, Elena [1 ,2 ]
Perez-Torrado, Roberto [1 ]
Gimeno-Alcaniz, Jose V. [1 ]
Matallana, Emilia [1 ,2 ]
机构：
[1] CSIC, Inst Agroquim & Tecnol Alimentos, Dept Biotecnol, E-46100 Valencia, Spain
[2] Univ Valencia, Dept Bioquim & Biol Mol, Valencia, Spain
来源：
FEMS YEAST RESEARCH | 2009年 / 9卷 / 01期
关键词：
Saccharomyces cerevisiae; stress resistance; acid trehalase; NEUTRAL TREHALASE; GENETIC-EVIDENCE; OSMOTIC-STRESS; ATH1; GENE; YEAST; METABOLISM; NTH1; THERMOTOLERANCE; DISRUPTION; TOLERANCE;
D O I：
10.1111/j.1567-1364.2008.00453.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The role of the acid trehalase encoded by the ATH1 gene in the yeast Saccharomyces cerevisiae is still unclear. In this work, we investigated the regulation of ATH1 transcription and found a clear involvement of the protein kinase Hog1p in the induction of this gene under severe stress conditions, such as high salt. We also detected changes in the acid trehalase activity and trehalose levels, indicating a role of the acid trehalase in intracellular trehalose mobilization. Finally, the growth analysis for different mutants in neutral and acid trehalases after high salt stress implicates acid trehalase activity in saline stress resistance.
引用
收藏
页码：52 / 62
页数：11
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