Simultaneous saccharification and cofermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis

被引:0
作者
McMillan, JD [1 ]
Newman, MM [1 ]
Templeton, DW [1 ]
Mohagheghi, A [1 ]
机构
[1] Natl Renewable Energy Lab, Biotechnol Ctr Fuels & Chem, Golden, CO 80401 USA
关键词
hydrolyzate conditioning; Zymomonas mobilis; enzymatic; saccharification; ethanol fermentation; SSCF; integrated testing;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Simultaneous saccharification and cofermentation (SSCF) was carried out at approximately 15% total solids using conditioned dilute-acid pretreated yellow poplar feedstock, an adapted variant of National Renewable Energy Laboratory (NREL) xylose-fermenting Zymomonas mobilis and either commercial or NREL-produced cellulase enzyme preparations. In 7 d, at a cellulase loading of 12 filter paper units per gram cellulose (FPU/g), the integrated system produced more than 3% w/v ethanol and achieved 54% conversion of all potentially available biomass sugars (total sugars) entering SSCF, A control SSCF employing Sigmacell cellulose and a commercial cellulase at an enzyme loading of 14 FPU/g achieved 65% conversion of total sugars to ethanol.
引用
收藏
页码:649 / 665
页数:17
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