Photo-protection by 3-bromo-4, 5-dihydroxybenzaldehyde against ultraviolet B-induced oxidative stress in human keratinocytes

被引：20

作者：

Hyun, Yu Jae ^{[1
]}

Piao, Mei Jing ^{[1
]}

Zhang, Rui ^{[1
]}

Choi, Yung Hyun ^{[2
]}

Chae, Sungwook ^{[3
]}

Hyun, Jin Won ^{[1
]}

机构：

[1] Jeju Natl Univ Sch Med, Cheju 690756, South Korea

[2] Dongeui Univ Coll Oriental Med, Dept Biochem, Pusan 614052, South Korea

[3] Korea Inst Oriental Med, Aging Res Ctr, Taejon 305811, South Korea

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2012年 / 83卷

基金：

新加坡国家研究基金会;

关键词：

3-Bromo-4; 5-dihydroxybenzaldehyde; Human HaCaT keratinocyte; Oxidative stress; Reactive oxygen species; Ultraviolet B; INDUCED APOPTOSIS; RED ALGA; STRAND BREAKS; UVB; DAMAGE; RADIATION; SKIN; DNA; BROMOPHENOLS; PHOTOPROTECTION;

D O I：

10.1016/j.ecoenv.2012.06.010

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Exposure of the skin to ultraviolet B (UVB) radiation leads to epidermal damage and the generation of reactive oxygen species (ROS) in skin cells, including keratinocytes. Therefore, the photo-protective effect of 3-bromo-4, 5-dihydroxybenzaldehyde (BDB) against UVB was assessed in human HaCaT keratinocytes exposed to UVB radiation in vitro. BDB restored cell viability, which decreased upon exposure to UVB radiation. BDB exhibited scavenging activity against 1, 1-dipheny1-2-picrylhydrazyl radicals, intracellular ROS induced by hydrogen peroxide (H2O2) or UVB radiation, the superoxide anion generated by the xanthine/xanthine oxidase system, and the hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2). Moreover, BDB absorbed UVB and decreased injury resulting from UVB-induced oxidative stress to lipids, proteins and DNA. Finally, BDB reduced UVB-induced apoptosis, as exemplified by fewer apoptotic bodies and a reduction in DNA fragmentation. Taken together, these results suggest that BDB protects human keratinocytes against UVB-induced oxidative stress by scavenging ROS and absorbing UVB rays, thereby reducing injury to cellular components. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：71 / 78

页数：8

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