Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis

被引：11

作者：

Zhang, Zhaoxia ^{[1
,2
]}

Hao, Shumei ^{[1
,2
]}

Zhu, Hongping ^{[1
,2
]}

Wang, Wenfeng ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2008年 / 92卷 / 02期

基金：

中国国家自然科学基金;

关键词：

1,4-naphthoquinone; lysozyme; laser flash photolysis; electron transfer; hydrogen atom abstraction; photosensitizer;

D O I：

10.1016/j.jphotobiol.2008.05.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photoprocesses of 1,4-naphthoquinone (NQ) and its photoreactions with lysozyme in acetonitrile/water (3: 1, v/v) solution were studied using 355 nm laser flash photolysis technique combined with electrophoresis and turbidimetric assay. The transient spectra of NQ were observed and the transient species were assigned. The electron transfer process from N,N,N ',N '-tetramethyl-p-phenylenediamine (TMPD) to NQ triplet state ((3)NQ(center dot)) was investigated and the rate constant was determined to be k(t1) = 2.0 x 10(10) M-1 s(-1). it has been found that (3)NQ(center dot) can abstract hydrogen atom from lysozyme with a rate constant of k(t2) = 2.4 x 10(10) M-1 s(-1). Furthermore, the results of steady-state analysis suggested that lysozyme can be damaged by NQ irradiated with UVA light influenced by the concentration of NQ and the gas saturated in the solution. The mechanisms of photosensitized damage of lysozyme were discussed. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：77 / 82

页数：6

共 50 条

[1] LASER FLASH-PHOTOLYSIS STUDIES ON HYDROGEN-ATOM ABSTRACTION FROM PHENOL BY TRIPLET NAPHTHOQUINONES IN ACETONITRILE [J].

AMADA, I ;

YAMAJI, M ;

SASE, M ;

SHIZUKA, H .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1995, 91 (17) :2751-2759

[2] Laser photolysis studies of electron transfer between triplet naphthoquinones and amines [J].

Amada, I ;

Yamaji, M ;

Tsunoda, S ;

Shizuka, H .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 1996, 95 (01) :27-32

[3] Time-resolved ESR study of spin exchange processes in the photoreduction of 9,10-anthraquinone-1,5-disulfonate [J].

Beckert, D ;

Fessenden, RW .

JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (05) :1622-1629

[4] Phototransformation of 1,4-naphthoquinone in aqueous solution [J].

Brahmia, O ;

Richard, C .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2003, 2 (10) :1038-1043

[5]

Bruce J. M., 1974, CHEM QUINONOID COM 1, P465

[6] THE ELECTROCHEMICAL DOMAIN OF PHOTOSYNTHESIS [J].

CROFTS, AR ;

WRAIGHT, CA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 726 (03) :149-185

[7] Repair of amino acid radicals of apolipoprotein B100 of low-density lipoproteins by flavonoids.: A pulse radiolysis study with quercetin and rutin [J].

Filipe, P ;

Morlière, P ;

Patterson, LK ;

Hug, GL ;

Mazière, JC ;

Mazière, C ;

Freitas, JP ;

Fernandes, A ;

Santus, R .

BIOCHEMISTRY, 2002, 41 (36) :11057-11064

[8] Electron transfer from aromatic amino acids to triplet quinones [J].

Goerner, Helmut .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2007, 88 (2-3) :83-89

[9] Photoreactions of p-benzo-, p-naphtho- and p-anthraquinones with ascorbic acid [J].

Goerner, Helmut .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2004, 3 (10) :933-938

[10] ASSAY OF LYSOZYME BY ITS LYTIC ACTION ON M-LYSODEIKTICUS CELLS [J].

GORIN, G ;

WANG, SF ;

PAPAPAVLOU, L .

ANALYTICAL BIOCHEMISTRY, 1971, 39 (01) :113-+

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