Preparation of sensitive monoclonal antibodies against triptolide and establishment of a rapid and cost-effective icELISA method for authentication of Tripterygium wilfordii and related products

被引：5

作者：

Duan, Yaping ^{[1
,2
]}

Liu, Congmin ^{[1
]}

Dou, Xiaowen ^{[1
]}

Wu, Liu ^{[1
,2
]}

Liu, Hao ^{[1
,2
]}

Shan, Linan ^{[1
,2
]}

Yang, Shihai ^{[2
]}

Luo, Jiaoyang ^{[1
]}

Yang, Meihua ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Key Lab Bioact Subst & Resources Utilizat Chinese, Inst Med Plant Dev, Minist Educ,Peking Union Med Coll, Beijing 100193, Peoples R China

[2] Jilin Agr Univ, Coll Tradit Chinese Med, Changchun 130118, Peoples R China

来源：

MICROCHEMICAL JOURNAL | 2020年 / 158卷

关键词：

Tripterygium wilfordii; Triptolide; Monoclonal antibody; IcELISA; Quality control; LINKED-IMMUNOSORBENT-ASSAY; HOOK-F; IN-VITRO; IDENTIFICATION; ALKALOIDS; EXTRACT;

D O I：

10.1016/j.microc.2020.105135

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Triptolide is a characteristic chemical component and quality marker of Tripterygium wilfordii Hook. F., a widely used traditional Chinese medicine with various pharmacological activities. Triptolide-14-succinate is a derivative of triptolide that can be combined with bovine serum albumin and ovalbumin to form an immunogen and coating antigen. Monoclonal antibody specific to triptolide-14-succinate conjugated with ovalbumin was produced and named mAb 2D10 1H8. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was established by using mAb 2D10 1H8. The 50% inhibition concentration and working range of triptolide for the icELISA were 0.28 and 0.09-0.87 mu g mL(-1), (based on 20-80% binding inhibition of mAb 2D10 1H8 to triptolide, R-2 = 0.9997, y = -0.571Logx + 0.246), respectively. The icELISA displayed crossreactivity values of 48.6, 20.5, 3.27, and < 1.00% for triptonide, triptophenolide, tripterifordin, and other analogs of triptolide, respectively. The average recovery of triptolide from the Tripterygium wilfordii samples as determined by icELISA ranged from 90.05 to 106.6%. Triptolide was detected in 38 batches of Tripterygium wilfordii samples by icELISA, which was validated by ultra-fast liquid chromatography combined with electrospray ionization tandem-mass spectrometry. In conclusion, the icELISA method established in the present study is applicable for rapid authentication of Tripterygium wilfordii and related products.

引用

页数：8

共 36 条

[1] Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo [J].

Antonoff, Mara B. ;

Chugh, Rohit ;

Borja-Cacho, Daniel ;

Dudeja, Vikas ;

Clawson, Kimberly A. ;

Skube, Steven J. ;

Sorenson, Brent S. ;

Saltzman, Daniel A. ;

Vickers, Selwyn M. ;

Saluja, Ashok K. .

SURGERY, 2009, 146 (02) :282-290

[2]

Cai J., 2012, J LIQ CHROMATOGR, V17, P4479

[3] General immunoassay for pyrethroids based on a monoclonal antibody [J].

Chen, Xiujin ;

Xu, Liguang ;

Ma, Wei ;

Liu, Liqiang ;

Kuang, Hua ;

Wang, Libing ;

Xu, Chuanlai .

FOOD AND AGRICULTURAL IMMUNOLOGY, 2014, 25 (03) :341-349

[4]

Chi Jie, 2015, Zhongguo Zhong Yao Za Zhi, V40, P1479

[5] Rapid identification of triptolide in Tripterygium wilfordii products by gold immunochromatographic assay [J].

Duan, Yaping ;

Luo, Jiaoyang ;

Liu, Congmin ;

Shan, Linan ;

Dou, Xiaowen ;

Yang, Shihai ;

Yang, Meihua .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2019, 168 :102-112

[6] Signal amplification using "spot on-a-chip" technology for the identification of proteins via MALDI-TOF MS [J].

Ekström, S ;

Ericsson, D ;

Önnerfjord, P ;

Bengtsson, M ;

Nilsson, J ;

Marko-Varga, G ;

Laurell, T .

ANALYTICAL CHEMISTRY, 2001, 73 (02) :214-219

[7] Comparison of Tripterygium wilfordii Hook F Versus Sulfasalazine in the Treatment of Rheumatoid Arthritis A Randomized Trial [J].

Goldbach-Mansky, Raphaela ;

Wilson, Mildred ;

Fleischmann, Roy ;

Olsen, Nancy ;

Silverfield, Joel ;

Kempf, Phillip ;

Kivitz, Alan ;

Sherrer, Yvonne ;

Pucino, Frank ;

Csako, Gyorgy ;

Costello, Rene ;

Pham, Tuyet Hang ;

Snyder, Christopher ;

van der Heijde, Desiree ;

Tao, Xuelian ;

Wesley, Robert ;

Lipsky, Peter E. .

ANNALS OF INTERNAL MEDICINE, 2009, 151 (04) :229-W51

[8] An enzyme-linked immunosorbent assay for aconitine-type alkaloids using an anti-aconitine monoclonal antibody [J].

Kido, Katsumi ;

Edakuni, Kyoko ;

Morinaga, Osamu ;

Tanaka, Hiroyuki ;

Shoyama, Yukihiro .

ANALYTICA CHIMICA ACTA, 2008, 616 (01) :109-114

[9] Molecular Molecular analyses of the Chinese herb Leigongteng (Tripterygium wilfordii Hook.f.) [J].

Law, Sue Ka-Yee ;

Simmons, Mark P. ;

Techen, Natascha ;

Khan, Ikhlas A. ;

He, Ming-Fang ;

Shaw, Pang-Chui ;

But, Paul Pui-Hay .

PHYTOCHEMISTRY, 2011, 72 (01) :21-26

[10] Triptolide: Progress on research in pharmacodynamics and toxicology [J].

Li, Xiao-Jiaoyang ;

Jiang, Zhen-Zhou ;

Zhang, Lu-yong .

JOURNAL OF ETHNOPHARMACOLOGY, 2014, 155 (01) :67-79

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