N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

被引：73

作者：

Zou, Yaozhong ^{[1
]}

Weis, William I. ^{[1
,2
]}

Kobilka, Brian K. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

PLOS ONE | 2012年 / 7卷 / 10期

基金：

美国国家卫生研究院;

关键词：

MUSCARINIC ACETYLCHOLINE-RECEPTOR; CRYSTAL-STRUCTURE; BETA(2)-ADRENERGIC RECEPTOR; ANTAGONIST; COMPLEX; GPCR;

D O I：

10.1371/journal.pone.0046039

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A highly crystallizable T4 lysozyme (T4L) was fused to the N-terminus of the beta(2) adrenergic receptor (beta(2)AR), a G-protein coupled receptor (GPCR) for catecholamines. We demonstrate that the N-terminal fused T4L is sufficiently rigid relative to the receptor to facilitate crystallogenesis without thermostabilizing mutations or the use of a stabilizing antibody, G protein, or protein fused to the 3rd intracellular loop. This approach adds to the protein engineering strategies that enable crystallographic studies of GPCRs alone or in complex with a signaling partner.

引用

页数：9

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