Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway

被引：24

作者：

Kung, Mei-Lang ^{[1
]}

Tsai, Han-En ^{[2
]}

Hu, Tsung-Hui ^{[4
]}

Kuo, Hsiao-Mei ^{[5
]}

Liu, Li-Fen ^{[6
]}

Chen, San-Cher ^{[2
,3
]}

Lin, Pey-Ru ^{[7
]}

Ma, Yi-Ling ^{[1
]}

Wang, E-Ming ^{[1
]}

Liu, Guei-Sheung ^{[8
]}

Liu, Jong-Kang ^{[1
]}

Tai, Ming-Hong ^{[1
,2
,3
]}

机构：

[1] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 804, Taiwan

[2] Natl Sun Yat Sen Univ, Inst Biomed Sci, Kaohsiung 804, Taiwan

[3] Natl Sun Yat Sen Univ, Ctr Neurosci, Kaohsiung 804, Taiwan

[4] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp,Dept Internal Med, Kaohsiung Med Ctr,Div Hepatogastroenter, Kaohsiung, Taiwan

[5] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Mitochondrial Res Unit,Kaohsiung Med Ctr, Kaohsiung, Taiwan

[6] I Shou Univ, Dept Biol Sci & Technol, Kaohsiung, Taiwan

[7] Natl Cheng Kung Univ, Inst Basic Med, Tainan 70101, Taiwan

[8] Univ Melbourne, OBrien Inst, Melbourne, Vic, Australia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 425卷 / 02期

关键词：

Cell migration; Hepatoma-derived growth factor; Podosome; PI3K; Akt; GENE-TRANSFER; ENDOTHELIAL-CELLS; MIGRATION; CANCER; PROLIFERATION; INVADOPODIA; INHIBITION;

D O I：

10.1016/j.bbrc.2012.07.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：169 / 176

页数：8

共 33 条

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