New Fluorescent Method (PLT-F) on Sysmex XN2000 Hematology Analyzer Achieved Higher Accuracy in Low Platelet Counting

Objectives: In thrombocytopenia, high accuracy and precision of low platelet count is essential for appropriate decisions. The recently introduced Sysmex XN2000 analyzer (Sysmex, Kobe, Japan) offers 3 methods for platelet counting: impedance (PLT-I), optical (PLT-O), and a new fluorescence method (PLT-F). The precision of the PLT-F method in blood samples with platelet counts less than 50 x 10(3)/mu L (50 x 10(9)/L) was investigated and compared with the ICSH CD61-ImmunoPLT reference method. For comparison, PLT-I and PLT-O were determined on the Sysmex XN2000 and Sysmex XE2100 analyzer. Methods: Blood samples with platelet counts less than 50 x 10(3)/mu L (50 x 10(9)/L) (n = 37) were analyzed on the Sysmex XN2000 and XE2100 analyzers. The CD61-ImmunoPLT method was performed on a Beckman Coulter FC-500 flow cytometer (Miami, FL). Results: At a platelet count of 20 x 10(3)/mu L (20 x 109/L), reproducibility for PLT-I, PLT-O, and PLT-F on the XN2000 demonstrated coefficients of variation of 9.3%, 8.5%, and 3.0%, respectively. Correlation between PLT-O on the XN2000 and XE2100 yielded an r value of more than 0.977. Linear regression analysis between the PLT-F and CD61-ImmunoPLT methods resulted in a PLT-F of 0.71*CD61-0.8 (r = 0.988). Linear regression between PLT-F and PLT-O on the XN2000 resulted in a PLT-F of 1.05*PLT-O-2 (r = 0.975), and using the transfusion threshold of 20 x 10(9)/L platelets resulted in a PLT-F of 0.90*PLT-O-0.4 (r = 0.956). Conclusions: The new PLT-F method demonstrated excellent results for reproducibility in samples with platelet counts less than 50 x 10(9)/L. PLT-F could be helpful in making better decisions for platelet transfusions.