Cytotoxic triterpenoid saponins fromThalictrum atriplex

Two new cycloartane glycosides, cycloatriosides A and B (1-2), and a new oleanolic acid glycoside, thaliatrioside A (3), along with7known triterpenoids (4-10) were isolated fromThalictrum atriplex. The structures of the new compounds were established as 3-O-beta-D-galactopyranosyl (20S, 24 R)-3 beta,16 beta,25,29-tetrahydroxy-20,24-epoxycycloartane-29-O-beta-D-glucopyranoside (1), 3-O-beta-D-glucopyranosyl-(1 -> 2)-alpha-arabinopyranosyl-3 beta,22 xi,30-trihydroxycycloart-24-en-21-oic acid alpha-L-arabinopyranosyl-(1 -> 6)-beta-D-glucopyranoside (2) and 3-O-[alpha-L-rhamnopyranosyl-(1 -> 3)-beta-D-xylopyranosyl-(1 -> 3)-alpha-L-rhamnopyranosyl-(1 -> 2)-alpha-L-arabinopyranosyl]-oleanolic acid 28-O-beta-D-glucopyranosyl ester (3) on the basis of extensive NMR and HR-ESI-MS analyses, along with acid hydrolysis. Their cytotoxic activities against human lung cancer cells A549 and human breast cancer cells MDA-MB-231 were evaluated using MTT method. Compound9showed cytotoxicity against MDA-MB-231 cell line with the IC(50)value of 72.53 +/- 1.08 mu M.