Extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Meyer) using commercial enzymes and high hydrostatic pressure

被引：17
作者：
Sunwoo, Hoon H. [1 ]
Kim, Chong-Tai [2 ]
Kim, Do-Yeon [2 ]
Maeng, Jin-Soo [2 ]
Cho, Chang-Won [2 ]
Lee, Soo-Jeong [3 ]
机构：
[1] Univ Alberta, Ctr Pharm & Hlth Res, Fac Pharm & Pharmaceut Sci, Katz Grp 3 142G, Edmonton, AB T6G 2E1, Canada
[2] Korea Food Res Inst, Songnam 463746, Gyeonggi, South Korea
[3] Bucheon Univ, Dept Food & Nutr, Puchon 420735, Gyeonggi, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 07期
关键词：
Enzyme; High hydrostatic pressure; HPLC; Ginsenoside; Panax ginseng; PERFORMANCE LIQUID-CHROMATOGRAPHY; ASSISTED EXTRACTION; ANTIOXIDANT; RG1; RE;
D O I：
10.1007/s10529-013-1182-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A combination of high hydrostatic pressure (HHP) and enzymatic hydrolysis (HHP-EH) was applied for the extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Myer). The highest yield of ginsenosides was obtained by using a mixture of three enzymes (Celluclast + Termamyl + Viscozyme) along with HHP (100 MPa, at 50 A degrees C for 12 h) in comparison to control samples (no enzymes, atmosphere pressure, P < 0.05). Total ginsenosides increased by 184 % while Rg1 + Rb1 increased by 273 %. Application of these conditions significantly increased total ginsenosides by 49 % and Rg1 + Rb1 by 103 % compared to HHP treatment alone (P < 0.05). The effect of HHP on increased yield of ginsenosides is likely due in part, to acceleration of enzyme activity. Thus HHP-EH significantly improves the extraction of ginsenosides from fresh ginseng roots.
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页码：1017 / 1022
页数：6
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