Sensitive analysis of aminoglycoside antibiotics via hyphenation of transient moving substitution boundary with field-enhanced sample injection in capillary electrophoresis

被引：31
作者：
Ge, Shuli [1 ]
Tang, Wanrong [1 ]
Han, Ruobing [1 ,2 ]
Zhu, Yan [1 ]
Wang, Qingjiang [1 ]
He, Pingang [1 ]
Fang, Yuzhi [1 ]
机构：
[1] E China Normal Univ, Dept Chem, Shanghai 200241, Peoples R China
[2] Shanghai Hongkou Ctr Dis Control & Prevent, Shanghai 200082, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1295卷
关键词：
Field-enhanced sample injection; Moving substitution boundary; Capillary zone electrophoresis; Amperometric detection; 18C6H4; Aminoglycoside antibiotics; ZONE-ELECTROPHORESIS; AMPEROMETRIC DETECTION; SOLVENT STACKING; CROWN-ETHER; ELECTROKINETIC CHROMATOGRAPHY; MICROCHIP ELECTROPHORESIS; ENANTIOMERIC SEPARATION; MICELLE COLLAPSE; TRACE ANALYSIS; PRECONCENTRATION;
D O I：
10.1016/j.chroma.2013.04.049
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel field-enhanced sample injection coupled with transient moving substitution boundary method in capillary electrophoresis was developed for aminoglycoside antibiotic (AG) analysis using 18-crown-6-tetracarboxylic acid (18C6H4) as a pseudostationary phase. Results indicated that the stacking mechanism of moving substitution boundary relied on the substitution reaction between 18C6H4-bonded AG complexes and Na+ at the substitution boundary. The stacking mechanism as well as important parameters governing pre-concentration and separation have been investigated in order to obtain maximum resolution and sensitivity. Under optimized conditions, using a sample prepared in a low-conductivity matrix, the limits of detection for streptomycin, neomycin, and kanamycin were 0.62, 5.9 and 8.6 nM (S/N = 3), respectively, and the detection sensitivities were improved 940-, 692-, and 415-fold, respectively. The method also gave accurate and reliable results in the analysis of AGs in river water samples. (C) 2013 Elsevier B.V. All rights reserved.
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收藏
页码：128 / 135
页数：8
相关论文
共 52 条
[41]   Chiral selectors in CE: Recent developments and applications [J].
Tsioupi, Despina A. ;
Stefan-van Staden, Raluca-Ioana ;
Kapnissi-Christodoulou, Constantina P. .
ELECTROPHORESIS, 2013, 34 (01) :178-204
[42]   Ultrasensitive Detection of Bacteria by Microchip Electrophoresis Based on Multiple-Concentration Approaches Combining Chitosan Sweeping, Field-Amplified Sample Stacking, and Reversed-Field Stacking [J].
Wang, Zhi-Fang ;
Cheng, Shuang ;
Ge, Shu-Li ;
Wang, Huan ;
Wang, Qing-Jiang ;
He, Pin-Gang ;
Fang, Yu-Zhi .
ANALYTICAL CHEMISTRY, 2012, 84 (03) :1687-1694
[43]   Recent advances in enrichment techniques for trace analysis in capillary electrophoresis [J].
Wen, Yingying ;
Li, Jinhua ;
Ma, Jiping ;
Chen, Lingxin .
ELECTROPHORESIS, 2012, 33 (19-20) :2933-+
[44]   Enantiomeric separation of chiral dipeptides by CE-ESI-MS employing a partial filling technique with chiral crown ether [J].
Xia, Shifei ;
Zhang, Lan ;
Lu, Minghua ;
Qiu, Bin ;
Chi, Yuwu ;
Chen, Guonan .
ELECTROPHORESIS, 2009, 30 (16) :2837-2844
[45]   Applications of a copper microparticle-modified carbon fiber microdisk array electrode for the simultaneous determination of aminoglycoside antibiotics by capillary electrophoresis [J].
Yang, WC ;
Yu, AM ;
Chen, HY .
JOURNAL OF CHROMATOGRAPHY A, 2001, 905 (1-2) :309-318
[46]   Rapid and selective micellar electrokinetic chromatography for simultaneous determination of amikacin kanamycin A and tobramcin with UV detection and application in drug formulations [J].
Yeh, HH ;
Lin, SJ ;
Ko, JY ;
Chou, CA ;
Chen, SH .
ELECTROPHORESIS, 2005, 26 (4-5) :947-953
[47]   Extremely Large Volume Electrokinetic Stacking of Cationic Molecules in MEKC by EOF Modulation with Strong Acids in Sample Solutions [J].
Zhang, Hui-ge ;
Zhu, Jin-hua ;
Qi, Sheng-da ;
Yan, Na ;
Chen, Xing-guo .
ANALYTICAL CHEMISTRY, 2009, 81 (21) :8886-8891
[48]   On-line sample concentration and determination of cationic alkaloids in human plasma by micelle to solvent stacking in capillary zone electrophoresis [J].
Zhang, Shuaihua ;
Ma, Ruiyang ;
Yang, Xiumin ;
Wang, Chun ;
Wang, Zhi .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2012, 906 :41-47
[49]   A novel isotachophoresis of cobalt and copper complexes by metal ion substitution reaction in a continuous moving chelation boundary [J].
Zhang, Wei ;
Chen, Jian-Feng ;
Fan, Liu-Yin ;
Cao, Cheng-Xi ;
Ren, Ji-Cun ;
Li, Si ;
Shao, Jing .
ANALYST, 2010, 135 (01) :140-148
[50]   Sweeping with Electrokinetic Injection and Analyte Focusing by Micelle Collapse in Two-Dimensional Separation via Integration of Micellar Electrokinetic Chromatography with Capillary Zone Electrophoresis [J].
Zhang, Zhaoxiang ;
Du, Xiuzhen ;
Li, Xuemei .
ANALYTICAL CHEMISTRY, 2011, 83 (04) :1291-1299
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