Sensitive analysis of aminoglycoside antibiotics via hyphenation of transient moving substitution boundary with field-enhanced sample injection in capillary electrophoresis

被引：31

作者：

Ge, Shuli ^{[1
]}

Tang, Wanrong ^{[1
]}

Han, Ruobing ^{[1
,2
]}

Zhu, Yan ^{[1
]}

Wang, Qingjiang ^{[1
]}

He, Pingang ^{[1
]}

Fang, Yuzhi ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200241, Peoples R China

[2] Shanghai Hongkou Ctr Dis Control & Prevent, Shanghai 200082, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1295卷

关键词：

Field-enhanced sample injection; Moving substitution boundary; Capillary zone electrophoresis; Amperometric detection; 18C6H4; Aminoglycoside antibiotics; ZONE-ELECTROPHORESIS; AMPEROMETRIC DETECTION; SOLVENT STACKING; CROWN-ETHER; ELECTROKINETIC CHROMATOGRAPHY; MICROCHIP ELECTROPHORESIS; ENANTIOMERIC SEPARATION; MICELLE COLLAPSE; TRACE ANALYSIS; PRECONCENTRATION;

D O I：

10.1016/j.chroma.2013.04.049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel field-enhanced sample injection coupled with transient moving substitution boundary method in capillary electrophoresis was developed for aminoglycoside antibiotic (AG) analysis using 18-crown-6-tetracarboxylic acid (18C6H4) as a pseudostationary phase. Results indicated that the stacking mechanism of moving substitution boundary relied on the substitution reaction between 18C6H4-bonded AG complexes and Na+ at the substitution boundary. The stacking mechanism as well as important parameters governing pre-concentration and separation have been investigated in order to obtain maximum resolution and sensitivity. Under optimized conditions, using a sample prepared in a low-conductivity matrix, the limits of detection for streptomycin, neomycin, and kanamycin were 0.62, 5.9 and 8.6 nM (S/N = 3), respectively, and the detection sensitivities were improved 940-, 692-, and 415-fold, respectively. The method also gave accurate and reliable results in the analysis of AGs in river water samples. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：128 / 135

页数：8

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