Epigenetics meets next-generation sequencing

被引：31

作者：

Park, Peter J. ^{[1
,2
]}

机构：

[1] Harvard Partners Ctr Genet & Genom, Boston, MA USA

[2] Childrens Hosp, HST Informat Program, Boston, MA 02115 USA

来源：

EPIGENETICS | 2008年 / 3卷 / 06期

关键词：

chromatin; histone modification; nucleosome; ChIP-seq; ChIP-chip;

D O I：

10.4161/epi.3.6.7249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Next-generation sequencing is poised to unleash dramatic changes in every area of molecular biology. In the past few years, chromatin immunoprecipitation (ChIP) on tiled microarrays (ChIP-chip) has been an important tool for genome-wide mapping of DNA-binding proteins or histone modifications. Now, ChIP followed by direct sequencing of DNA fragments (ChIP-seq) offers superior data with less noise and higher resolution and is likely to replace ChIP-chip in the near future. We will describe advantages of this new technology and outline some of the issues in dealing with the data. ChIP-seq generates considerably larger quantities of data and the most challenging aspect for investigators will be computational and statistical analysis necessary to uncover biological insights hidden in the data.

引用

页码：318 / 321

页数：4

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