A Self-Calibrating Bipartite Viscosity Sensor for Mitochondria

被引：422

作者：

Yang, Zhigang ^{[1
]}

He, Yanxia ^{[1
]}

Lee, Jae-Hong ^{[1
]}

Park, Nayoung ^{[1
]}

Suh, Myungkoo ^{[2
]}

Chae, Weon-Sik ^{[3
]}

Cao, Jianfang ^{[5
]}

Peng, Xiaojun ^{[5
]}

Jung, Hyosung ^{[1
]}

Kang, Chulhun ^{[4
]}

Kim, Jong Seung ^{[1
]}

机构：

[1] Korea Univ, Dept Chem, Seoul 136701, South Korea

[2] Kyung Hee Univ, Dept Appl Chem, Yongin 446701, South Korea

[3] Korea Basic Sci Inst, Gangneung Ctr, Kangnung 210702, South Korea

[4] Kyung Hee Univ, Sch East West Med Sci, Yongin 446701, South Korea

[5] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 24期

基金：

新加坡国家研究基金会;

关键词：

ENERGY-TRANSFER CASSETTES; MOLECULAR ROTORS; BODIPY DYES; LIVE CELLS; FLUORESCENT; MODULATION; EMISSION; ARRAYS;

D O I：

10.1021/ja403851p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.

引用

页码：9181 / 9185

页数：5

共 40 条

[1] Water-soluble through-bond energy transfer cassettes for intracellular imaging [J].