Comparative study of the M.BstF5I-1 and M.BstF5I-3 DNA methyltransferases from the Bacillus stearothermophilus F5 restriction-modification system

被引：1
作者：
Netesova, NA [1 ]
Golikova, LN
Ovetchkina, LG
Evdokimov, AA
Malygin, EG
Gololobova, NS
Gonchar, DA
Degtyarev, SK
机构：
[1] VECTOR, State Res Ctr Virol & Biotechnol, Koltsov 630559, Russia
[2] Russian Acad Med Sci, Siberian Div, Inst Mol Biol, Novosibirsk 630117, Russia
来源：
MOLECULAR BIOLOGY | 2002年 / 36卷 / 01期
基金：
俄罗斯基础研究基金会;
关键词：
Bacillus stearothermophilus; DNA methyltransferases; restriction-modification systems;
D O I：
10.1023/A:1014267027786
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The BstF5I restriction-modification system from Bacillus stearothermophilus F5, unlike all known restriction-modification systems, contains three genes encoding DNA methyltransferases. In addition to revealing two DNA methylases responsible for modification of adenine in different DNA strands, it has been first shown that one bacterial cell has two DNA methylases, M.BstF5I-1 and M.BstF5I-3, with similar substrate specificity. The boundaries of the gene for DNA methyltransferase M.BstF5I-1 have been verified. The bstF51M-1 gene was cloned in pJW and expressed in Escherichia coli. Homogeneous samples of M.BstF5I-1 and M.BstF5I-3 were obtained by chromatography with different sorbents. The main kinetic parameters have been determined for M.BstF5I-1 and M.BstF5I-3, both modifying adenine in the recognition site 5'-GGATG-3'.
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收藏
页码：110 / 116
页数：7
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