Nociception- and anxiety-like behavior in rats submitted to different periods of restraint stress

被引:119
作者
Gameiro, GH
Gameiro, PH
Andrade, AD
Pereira, LF
Arthuri, MT
Marcondes, FK
Veiga, MCD
机构
[1] Univ Estadual Campinas, Fac Dent, Dept Physiol Sci, UNICAMP, BR-13414900 Piracicaba, SP, Brazil
[2] UFPEL, Inst Biol, Dept Microbiol & Parasitol, Pelotas, Brazil
基金
巴西圣保罗研究基金会;
关键词
stress; anxiety; temporomandibular disorders; facial pain;
D O I
10.1016/j.physbeh.2005.12.007
中图分类号
B84 [心理学];
学科分类号
04 ; 0402 ;
摘要
The aim of this study was to evaluate the effect of acute, sub-chronic and chronic stress on nociception induced by formalin injection in rats' temporomandibular joint (TMJ). It was evaluated the relation between blood levels of adrenocorticotropin, corticosterone, the levels of anxiety and nociceptive responses recorded after different stress protocols. Animals were initially submitted to acute restraint stress (15; 30 min and 1 h), or exposed to sub-chronic (3 days-1 h/day) or chronic stress (40 days-1 h/day). Then, animals were (1) killed immediately to collect blood for hormonal determinations; or (2) submitted to the elevated plus-maze to evaluate anxiety; or (3) submitted to the TMJ formalin test to evaluate nociception. It was also evaluated the role of serotoninergic and opioid systems in nociceptive changes induced by stress. For this, the serotonin-selective reuptake inhibitor (fluoxetine 10 mg/kg) and the opioid agonist (morphine 1-5 mg/kg) were administered before the nociception test. All stress protocols significantly raised the levels of ACTH or corticosterone, as well as the anxiety behavior. In relation to nociception, the chronic stressed animals showed an increase in nociceptive responses (hyperalgesia). In this group, there was a reduction in the morphine analgesic effects, suggesting dysfunction in the endogenous opioid system. Fluoxetine had an analgesic effect in both stressed and control groups, although this effect was more evident in the stressed group. It was concluded that stress-induced hyperalgesia may result from changes in the serotoninergic and opioid systems, which can explain, at least in part, the important link between stress and orofacial pain. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:643 / 649
页数:7
相关论文
共 55 条
[1]   Evaluation of the anti-inflammatory and anti-nociceptive effects of different antidepressants in the rat [J].
Abdel-Salam, OME ;
Nofal, SM ;
El-Shenawy, SM .
PHARMACOLOGICAL RESEARCH, 2003, 48 (02) :157-165
[2]   Comparative study in the rat of the actions of different types of stress on the release of 5-HT in raphe nuclei and forebrain areas [J].
Adell, A ;
Casanovas, JM ;
Artigas, F .
NEUROPHARMACOLOGY, 1997, 36 (4-5) :735-741
[3]   CAN ANXIETY HELP US TOLERATE PAIN [J].
ALABSI, M ;
ROKKE, PD .
PAIN, 1991, 46 (01) :43-51
[4]   Behavioural and hormonal effects of restraint stress and formalin test in male and female rats [J].
Aloisi, AM ;
Ceccarelli, I ;
Lupo, C .
BRAIN RESEARCH BULLETIN, 1998, 47 (01) :57-62
[5]   PITUITARY-GLAND MEDIATES ACUTE AND CHRONIC PAIN RESPONSIVENESS IN STRESSED AND NON-STRESSED RATS [J].
AMIR, S ;
AMIT, Z .
LIFE SCIENCES, 1979, 24 (05) :439-448
[6]  
[Anonymous], OPIATES ENDOGENOUS O
[7]   Mechanisms of the Effects of Adrenocorticotropic Hormone on Pain Sensitivity in Rats [J].
A. I. Bogdanov ;
N. I. Yarushkina .
Neuroscience and Behavioral Physiology, 2003, 33 (8) :795-798
[8]   A PERCEPTUAL-DEFENSIVE-RECUPERATIVE MODEL OF FEAR AND PAIN [J].
BOLLES, RC ;
FANSELOW, MS .
BEHAVIORAL AND BRAIN SCIENCES, 1980, 3 (02) :291-301
[9]   FACTORS AFFECTING RESTRAINT STRESS-INDUCED POTENTIATION OF MORPHINE ANALGESIA [J].
CALCAGNETTI, DJ ;
HOLTZMAN, SG .
BRAIN RESEARCH, 1990, 537 (1-2) :157-162
[10]   TIME-COURSE OF HYPOTHALAMIC CRH AND PITUITARY ACTH CONTENTS, AND PITUITARY-RESPONSIVENESS TO CRH STIMULATION AFTER BILATERAL ADRENALECTOMY [J].
CASTRO, M ;
FIGUEIREDO, F ;
MOREIRA, AC .
HORMONE AND METABOLIC RESEARCH, 1995, 27 (01) :10-15