Wounding activates a 47 kDa MAP kinase in Catharanthus roseus (L.) G. Don

被引:0
作者
Raina, Susheel Kumar [1 ,2 ]
机构
[1] Natl Inst Plant Genome Res, JNU Campus,Aruna Asif Ali Marg, New Delhi 110067, India
[2] ICAR Cen Inst Temperate Hort, Srinagar 190007, Jammu & Kashmir, India
关键词
Madagascar periwinkle; MAP Kinase; Wounding; PROTEIN-KINASE; INDOLE ALKALOIDS; STRESS; ACID; PHOSPHORYLATION; PLANTS; MECHANISMS; PERIWINKLE; TOLERANCE; DEPLETION;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Catharanthus roseus (L.) G. Don. is an important medicinal plant (Fam. Apocynaceae) known for its alkaloids that are accumulated in response to various biotic and abiotic stresses including wounding. Mitogen activated protein (MAP) kinases are important components of cellular signaling system transducing these stress stimuli into intracellular responses by phosphorylation of downstream transcription factors, regulators or inhibitors leading to expression of stress responsive genes. Here, we report the activation of a 47 kDa MAP Kinase in C. roseus in response to wounding. The immunoprecipitation coupled with in-gel kinase assay revealed tyrosine phosphorylation of the protein. Partial purification of the MAP kinase was also attempted. We observed a size variation for the kinase when purified using different schemes.
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页码:107 / 112
页数:6
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