Gene expression profiles and functional characterization of human immortalized endometriotic epithelial and stromal cells

被引：78

作者：

Banu, Sakhila K. ^{[1
]}

Lee, JeHoon ^{[1
]}

Starzinski-Powitz, Anna ^{[2
]}

Arosh, Joe A. ^{[1
]}

机构：

[1] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Integrat Biosci, Reprod Endocrinol & Cell Signaling Lab, College Stn, TX 77843 USA

[2] Goethe Univ Frankfurt, Inst Anthropol & Humangenet Biol, Frankfurt, Germany

来源：

FERTILITY AND STERILITY | 2008年 / 90卷 / 04期

关键词：

endometriosis; immortalized; endometriotic cells; COX-2; PGE2; MPP2 and MMP9; endometriotic cell migration and invasion;

D O I：

10.1016/j.fertnstert.2007.07.1358

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To determine whether immortalized endometriotic cells can be used as potential models to unravel pathophysiology/pathogenesis of endometriosis in humans. Design: In vitro study. Human immortalized endometriotic epithelial and stromal cells. Setting: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University. Patient(s): Not applicable. Intervention(s): None. Main Outcome Measure(s): Gene expression pattern and prostaglandin E-2 production and activity of matrix metalloproteinase 2 and matrix metalloproteinase 9. Result(s): Several genes associated with estrogen and progesterone biosynthesis and signaling, cell cycle regulation, extracellular matrix degradation, angiogenesis, cell growth and survival, cytokine production, and prostaglandin E-2 biosynthesis, transport, and signaling were expressed in human immortalized endometriotic cells. These endometriotic epithelial and stromal. cells exhibited migrating and invading potential, produced a high quantity of prostaglandin E-2, and showed higher matrix metalloproteinase 2 and matrix metalloprotemase 9 activity compared to normal endometrial, epithelial, and stromal cells. Conclusion(s): Human immortalized endometriotic epithelial and stromal. cells could be used as ideal models to study the molecular and cellular aspects of endometriosis in humans.

引用

页码：972 / 987

页数：16

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