Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis

被引：41

作者：

Mohanty, S. ^{[1
]}

Panda, M. K. ^{[1
]}

Subudhi, E. ^{[1
]}

Nayak, S. ^{[1
]}

机构：

[1] Siksha O Anusandhan Univ, Ctr Biotechnol, Bhubaneswar 751030, Orissa, India

来源：

BIOLOGIA PLANTARUM | 2008年 / 52卷 / 04期

关键词：

DNA content; tissue culture; wild turmeric;

D O I：

10.1007/s10535-008-0153-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Callus cultures initiated from shoot base explants of Curcuma aromatica Salisb. were maintained on Murashige and Skoog (MS) media supplemented with 2 mg dm(-3) 2,4-dichlorophenoxyacetic acid alone or with 0.5 mg dm(-3) kinetin. Plantlets were regenerated from 60 and 180-d-old callus on MS media supplemented with 3 mg dm(-3) benzyladenine and 0.5 mg dm(-3) alpha-naphthalene acetic acid. Approximately 8-10 plantlets were produced after 30-40 d of culture per 50 mg of callus inoculated. Out of 113 regenerants analyzed 85 plants were exclusively diploid and 28 were predominantly diploid revealing presence of polyploid nuclei. Frequency of polyploid cells were more in regenerants obtained from 180-d-old callus then from 6-d-old callus which might be attributed to the ageing of callus.

引用

页码：783 / 786

页数：4

共 21 条

[1] Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis [J].