Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline

被引：15

作者：

Zhang, Yongli ^{[1
]}

Wang, Xiangsheng ^{[1
]}

Fang, Wei ^{[1
]}

Cai, Xiaoyan ^{[1
]}

Chu, Fujiang ^{[2
]}

Liao, Xiangwen ^{[3
]}

Lu, Jiazheng ^{[3
]}

机构：

[1] Guangdong Pharmaceut Univ, Dept Biol, Sch Basic Courses, Guangzhou 510006, Guangdong, Peoples R China

[2] Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Guangdong, Peoples R China

[3] Guangdong Pharmaceut Univ, Dept Chem, Sch Pharm, Guangzhou 510006, Guangdong, Peoples R China

来源：

BIOINORGANIC CHEMISTRY AND APPLICATIONS | 2013年 / 2013卷

基金：

中国国家自然科学基金;

关键词：

TRANSITION-METAL-COMPLEXES; DNA-BINDING; CELL-DEATH; APOPTOSIS; VANADIUM; CANCER; CLEAVAGE; COPPER(II); CYTOTOXICITY;

D O I：

10.1155/2013/437134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, H-1 NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (Delta Psi m). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the Delta Psi m, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1.

引用

页数：14

共 46 条

[1]

[Anonymous], 1998, ACS SYM SER, DOI [DOI 10.1021/BK-1998-0711.CH001, 10.1021/bk-1998-0711.ch001]

[2] Oxovanadium(IV) and oxovanadium(IV)-barium(II) complexes with heterotopic macrocyclic ligands based on isothiosemicarbazide [J].