An automated, highly sensitive LC-MS/MS assay for the quantification of the opiate antagonist naltrexone and its major metabolite 6β-naltrexol in dog and human plasma

被引：20

作者：

Clavijo, Claudia ^{[2
]}

Bendrick-Peart, Jamie ^{[1
,2
]}

Zhang, Yan Ling ^{[2
]}

Johnson, Gillian ^{[2
]}

Gasparic, Antje ^{[3
]}

Christians, Uwe ^{[1
,2
]}

机构：

[1] Eurofins Medinet, Aurora, CO 80045 USA

[2] Univ Colorado, Hlth Sci Ctr, Dept Anesthesiol, Aurora, CO USA

[3] Elbion AG, Radebeul, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 874卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

Naltrexone; 6; beta-Naltrexol; LC-MS/MS; Column switching; Dog plasma; Human plasma;

D O I：

10.1016/j.jchromb.2008.08.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To support animal studies and clinical pharmacokinetic trials, we developed and validated an automated, specific and highly sensitive LC-MS/MS method for the quantification of naltrexone and 6 beta-naltrexol in the same run. In human plasma, the assay had a lower limit of quantitation of only 5 pg/mL This was of critical importance to follow naltrexone pharmacokinetics during its terminal elimination phase. The assay had the following key performance characteristics for naltrexone in human plasma: range of reliable quantification: 0.005-100 ng/mL (r(2) > 0.99), inter-day accuracy (0.03 ng/mL): 103.7% and inter-day precision: 10.1%. There were no ion suppression, matrix interferences or carry-over. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：33 / 41

页数：9

共 20 条

[1]

Centre for Drug Evaluation and Research (CDER), 2001, GUID IND BIOAN METH

[2] Quantification of naltrexone and 6,β-naltrexol in plasma and milk using gas chromatography-mass spectrometry -: Application to studies in the lactating sheep [J].