Complete Monosaccharide Analysis by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

被引：137

作者：

Zhang, Zhenqing ^{[1
]}

Khan, Nazeer M. ^{[1
]}

Nunez, Karen M. ^{[1
]}

Chess, Edward K. ^{[1
]}

Szabo, Christina M. ^{[1
]}

机构：

[1] Baxter Healthcare Corp, Round Lake, IL 60073 USA

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 09期

关键词：

ACID; GLYCOPROTEINS; SULFATE; PROTEOGLYCANS; HYDROLYSIS; ASSAY; PH;

D O I：

10.1021/ac300176z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high-performance anion-exchange chromatography method with pulsed amperometric detection was initially developed to analyze acidic sugars including different. uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method has a limit of quantitation of similar to 12.5 x 10(-3) nmol.. for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety of complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed.

引用

页码：4104 / 4110

页数：7

共 41 条

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