A Novel Real-Time PCR Method for Determination and Quantification of Each Cytomegalovirus Glycoprotein H Subtype in Clinical Samples

被引:9
作者
Ikuta, Kazufumi [1 ]
Ishioka, Ken [1 ]
Sato, Yuka [1 ]
Imamura, Takashi [2 ]
Asano, Kimisato [3 ]
Koyano, Shin [4 ]
Inoue, Naoki [5 ]
Suzutani, Tatsuo [1 ]
机构
[1] Fukushima Med Univ, Sch Med, Dept Microbiol, Fukushima, Japan
[2] Fukushima Med Univ, Sch Med, Dept Pediat, Fukushima, Japan
[3] Fukushima Med Univ, Sch Med, Dept Obstet, Fukushima, Japan
[4] Asahikawa Med Univ, Sch Med, Dept Pediat, Asahikawa, Hokkaido, Japan
[5] Natl Inst Infect Dis, Dept Virol, Tokyo, Japan
关键词
INFECTION; STRAIN; ANTIBODY; IMMUNITY; ASSOCIATION; POPULATION; PREVALENCE; CHILDREN; VIRUS; WOMEN;
D O I
10.1128/JCM.06032-11
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To investigate reinfection in patients with congenital cytomegalovirus (CMV) infection, we established a CMV subtype-specific real-time quantitative PCR method targeting the CMV gH epitope region that can be used for evaluating pathogenic CMV strains in cases of mixed CMV infection.
引用
收藏
页码:499 / 501
页数:3
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