A Novel Real-Time PCR Method for Determination and Quantification of Each Cytomegalovirus Glycoprotein H Subtype in Clinical Samples

被引：9

作者：

Ikuta, Kazufumi ^{[1
]}

Ishioka, Ken ^{[1
]}

Sato, Yuka ^{[1
]}

Imamura, Takashi ^{[2
]}

Asano, Kimisato ^{[3
]}

Koyano, Shin ^{[4
]}

Inoue, Naoki ^{[5
]}

Suzutani, Tatsuo ^{[1
]}

机构：

[1] Fukushima Med Univ, Sch Med, Dept Microbiol, Fukushima, Japan

[2] Fukushima Med Univ, Sch Med, Dept Pediat, Fukushima, Japan

[3] Fukushima Med Univ, Sch Med, Dept Obstet, Fukushima, Japan

[4] Asahikawa Med Univ, Sch Med, Dept Pediat, Asahikawa, Hokkaido, Japan

[5] Natl Inst Infect Dis, Dept Virol, Tokyo, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 02期

关键词：

INFECTION; STRAIN; ANTIBODY; IMMUNITY; ASSOCIATION; POPULATION; PREVALENCE; CHILDREN; VIRUS; WOMEN;

D O I：

10.1128/JCM.06032-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To investigate reinfection in patients with congenital cytomegalovirus (CMV) infection, we established a CMV subtype-specific real-time quantitative PCR method targeting the CMV gH epitope region that can be used for evaluating pathogenic CMV strains in cases of mixed CMV infection.

引用

页码：499 / 501

页数：3

共 20 条

[1]

Ahlfors K, 2001, PEDIATRICS, V107, P1227, DOI 10.1542/peds.107.5.1227

[2] Intrauterine transmission of cytomegalovirus to infants of women with preconceptional immunity. [J].

Boppana, SB ;

Rivera, LB ;

Fowler, KB ;

Mach, M ;

Britt, WJ .

NEW ENGLAND JOURNAL OF MEDICINE, 2001, 344 (18) :1366-1371

[3] Congenital cytomegalovirus infection in a highly seropositive semi-urban population in India [J].

Dar, Lalit ;

Pati, Sunil Kumar ;

Patro, A. Raj Kumar ;

Deorari, Ashok Kumar ;

Rai, Sanjay ;

Kant, Shashi ;

Broor, Shobha ;

Fowler, Karen B. ;

Britt, William J. ;

Boppana, Suresh B. .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2008, 27 (09) :841-843

[4] Maternal immunity and prevention of congenital cytomegalovirus infection [J].

Fowler, KB ;

Stagno, S ;

Pass, RF .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2003, 289 (08) :1008-1011

[5] Identification of a highly conserved region in the human cytomegalovirus glycoprotein H gene and design of molecular diagnostic methods targeting the region [J].

Fukushima, Eiko ;

Ishibashia, Kei ;

Kaneko, Hisatoshi ;

Nishimura, Hidekazu ;

Inoue, Naoki ;

Tokumoto, Tadahiko ;

Tanabe, Kazunari ;

Ishioka, Ken ;

Ogawa, Hiroshi ;

Suzutani, Tatsuo .

JOURNAL OF VIROLOGICAL METHODS, 2008, 151 (01) :55-60

[6] Congenital cytomegalovirus infection: Review of the epidemiology and outcome [J].

Gaytant, MA ;

Steegers, EAP ;

Semmekrot, BA ;

Merkus, HMMW ;

Galama, JMD .

OBSTETRICAL & GYNECOLOGICAL SURVEY, 2002, 57 (04) :245-256

[7] Association of the outcome of renal transplantation with antibody response to cytomegalovirus strain - Specific glycoprotein H epitopes [J].

Ishibashi, Kei ;

Tokumoto, Tadahiko ;

Tanabe, Kazunari ;

Shirakawa, Hiroki ;

Hashimoto, Koichi ;

Kushida, Nobuhiro ;

Yanagida, Tomohiko ;

Inoue, Naoki ;

Yamaguchi, Osamu ;

Toma, Hiroshi ;

Suzutani, Tatsuo .

CLINICAL INFECTIOUS DISEASES, 2007, 45 (01) :60-67

[8] Strain-specific seroepidemiology and reinfection of cytomegalovirus [J].

Ishibashi, Kei ;

Tokumoto, Tadahiko ;

Shirakawa, Hiroki ;

Hashimoto, Koichi ;

Kushida, Nobuhiro ;

Yanagida, Tomohiko ;

Shishido, Keiichi ;

Aikawa, Ken ;

Yamaguchi, Osamu ;

Toma, Hiroshi ;

Tanabe, Kazunari ;

Suzutani, Tatsuo .

MICROBES AND INFECTION, 2008, 10 (12-13) :1363-1369

[9] Screening for congenital cytomegalovirus infection using newborn urine samples collected on filter paper: feasibility and outcomes from a multicentre study [J].

Koyano, Shin ;

Inoue, Naoki ;

Oka, Akira ;

Moriuchi, Hiroyuki ;

Asano, Kimisato ;

Ito, Yushi ;

Yamada, Hideto ;

Yoshikawa, Tetsushi ;

Suzutani, Tatsuo .

BMJ OPEN, 2011, 1 (01)

[10]

KRECH U, 1973, B WORLD HEALTH ORGAN, V49, P103

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